Jiangsu Provincial Key Lab for Organic Slid Waste Utilization, Nanjing Agricultural University, Nanjing 210095, PR China.
Bioresour Technol. 2010 Dec;101(23):9264-71. doi: 10.1016/j.biortech.2010.07.052. Epub 2010 Jul 17.
Effect of metal ions (Mg(2+), Zn(2+) and Fe(3+)) on the production of fusaricidin-type antifungal compounds by Paenibacillus polymyxa SQR-21 was studied in liquid culture. First, one-factor; three-level experiments were conducted to find out optimal concentrations of each metal ion for maximum production of fusaricidins. Later, three-factor; five-level experiments were performed and a quadratic predictive model was developed using response surface methodology (RSM). The results indicated that Fe(3+) and Mg(2+) positively affected the growth of P. polymyxa as determined by measuring the OD(600) of the liquid culture. The production of fusaricidin-type antifungal compounds was significantly inhibited by Zn(2+) (P=0.0114) and increased by Mg(2+) (P=0.0051) but the effect of Fe(3+) (P=0.2157) was non-significant. However, a synergistic positive effect of Mg(2+) and Fe(3+) on the production of antifungal compounds was observed. This study sheds lights on the pertinent effects of the individual and combined metal ions on the production of fusaricidins in P. polymyxa. It provides the key information for optimization of the metal ions in the fermentation media to achieve the maximum antibiotic production in this strain.
研究了金属离子（Mg(2+)、Zn(2+)和 Fe(3+)）对多粘类芽孢杆菌 SQR-21 产生 Fusaricidin 型抗真菌化合物的影响。首先，通过单因素三水平实验确定了每种金属离子的最佳浓度，以获得 Fusaricidins 的最大产量。然后，进行了三因素五水平实验，并使用响应面法（RSM）建立了二次预测模型。结果表明，Fe(3+)和 Mg(2+)通过测量液体培养物的 OD(600)，对多粘类芽孢杆菌的生长有积极影响。Zn(2+)（P=0.0114）显著抑制 Fusaricidin 型抗真菌化合物的产生，而 Mg(2+)（P=0.0051）则增加了其产生，但 Fe(3+)（P=0.2157）的影响不显著。然而，Mg(2+)和 Fe(3+)对抑菌化合物的产生存在协同的积极影响。本研究揭示了单个和组合金属离子对多粘类芽孢杆菌产生 Fusaricidins 的相关影响。它为优化发酵培养基中的金属离子以实现该菌株抗生素产量最大化提供了关键信息。
