Department of Energy Convergence and Environmental Engineering, Chosun University, 309 Pilmun-daero, Gwangju, 61452, Republic of Korea.
Green Chemistry and Materials Group, Korea Institute of Industrial Technology, 89 Yangdaegiro-gil, Cheonan-si, 31056, Chungcheongnam-do, Republic of Korea.
Appl Biochem Biotechnol. 2020 Feb;190(2):712-720. doi: 10.1007/s12010-019-03121-y. Epub 2019 Sep 2.
In this study, we investigated the potential of Paenibacillus kribbensis CU01 in producing fusaricidin, a strong antifungal substance, via optimization of metal ions and carbon and nitrogen source, and continuous fermentation. In the cultivation of a 2-l batch, maximal production of fusaricidins (581 mg l) was achieved in a modified M9 medium containing metal ions, 10 g l glucose, and 1 g l ammonium chloride. Most of glucose was consumed at a rate of 0.74 g l h within 24 h and fusaricidin production began 15 h after batch cultivation. Continuous fermentation was performed using a 7-l fermenter with 2-l working volume of modified M9 medium containing 10 g l glucose, 1 × 10 M FeSO, and 1 × 10 M MnCl. After 24 h of the start of cultivation, fresh M9 medium was continuously supplied at a flow rate of 2.5 ml min, and simultaneously, the same amount of cell culture broth was removed. In a continuous system, the highest fusaricidin concentration (579 mg l) was obtained using a dilution rate of 0.075 h with an average productivity of 10.4 mg l h for 24 to 72 h of incubation. Based on these results, it was found that fusaricidin production using P. kribbens CU01 strain increased by at least 28 times the values reported in previous studies.
在本研究中，我们通过优化金属离子和碳氮源以及连续发酵，研究了蜡状芽孢杆菌 CU01 产生 Fusaricidin 的潜力。在 2 升分批培养中，在含有金属离子、10g/L 葡萄糖和 1g/L 氯化铵的改良 M9 培养基中， Fusaricidin 的最大产量达到 581mg/L。大多数葡萄糖在 24 小时内以 0.74g/L/h 的速度消耗，在分批培养后 15 小时开始产生 Fusaricidin。在装有 7 升发酵罐的连续发酵中，使用 2 升工作体积的改良 M9 培养基进行连续发酵，其中含有 10g/L 葡萄糖、1×10MFeSO 和 1×10MMnCl。培养开始后 24 小时，以 2.5ml/min 的流速连续供应新鲜 M9 培养基，同时取出等量的细胞培养液。在连续系统中，以 0.075h 的稀释率获得最高 Fusaricidin 浓度（579mg/L），在 24 至 72 小时的孵育期间，平均生产力为 10.4mg/L/h。基于这些结果，发现与以前的研究报告相比，使用蜡状芽孢杆菌 CU01 菌株生产 Fusaricidin 的产量至少增加了 28 倍。
