抗小鼠肝炎病毒单克隆抗体F(ab')2片段的保护作用

Protective effect of the F(ab')2 fragments of monoclonal antibodies to mouse hepatitis virus.

作者信息

Nakanaga K, Yamanouchi K, Fujiwara K

机构信息

Department of Animal Pathology, University of Tokyo.

出版信息

Adv Exp Med Biol. 1987;218:365-71. doi: 10.1007/978-1-4684-1280-2_45.

DOI:10.1007/978-1-4684-1280-2_45

PMID:2829547

Abstract

The F(ab')2 fragments were prepared from three monoclonal antibodies (MAbs) reactive with either peplomer glycoprotein (E2) or nucleocapsid protein (NP) of a low-virulence mouse hepatitis virus (MHV), MHV-NuU. All the three MAbs could protect mice from challenge infection with virulent MHV-2, whereas only one of the anti-E2 MAbs was capable of neutralizing the virus in vitro. The F(ab')2 fragment of neutralizing anti-E2 MAb was shown to protect mice from challenge infection, but those of non-neutralizing anti-E2 and anti-NP MAbs were not protective.

摘要

F(ab')2片段由三种单克隆抗体（MAb）制备而成，这些单克隆抗体与低毒力小鼠肝炎病毒（MHV）MHV-NuU的包膜糖蛋白（E2）或核衣壳蛋白（NP）发生反应。所有这三种单克隆抗体都能保护小鼠免受强毒力MHV-2的攻击感染，而只有一种抗E2单克隆抗体能够在体外中和该病毒。具有中和作用的抗E2单克隆抗体的F(ab')2片段显示能保护小鼠免受攻击感染，但不具有中和作用的抗E2和抗NP单克隆抗体的F(ab')2片段则没有保护作用。