一种用于区分感染传染性胃肠炎病毒（TGEV）或与TGEV相关的猪呼吸道冠状病毒的猪血清抗体的竞争性抑制酶联免疫吸附测定。
A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus.
作者信息
Callebaut P, Pensaert M B, Hooyberghs J
机构信息
Laboratory of Virology, Faculty of Veterinary Medicine, State University of Ghent, Belgium.
出版信息
Vet Microbiol. 1989 May;20(1):9-19. doi: 10.1016/0378-1135(89)90003-5.
Abstract
A competitive inhibition ELISA was developed to detect non-neutralizing antibodies to the peplomer protein of transmissible gastroenteritis virus (TGEV) in porcine sera using a monoclonal antibody as an indicator. It was demonstrated that field strains of the TGEV-related porcine respiratory coronavirus (PRCV) did not induce this antibody, whereas the Miller strain and field strains of TGEV did. The sensitivity of the competitive inhibition ELISA appeared to be similar to that of the virus neutralization (VN) test. The test enables differentiation of pigs which were previously infected with TGEV or PRCV and which cannot be distinguished by the classical anti-TGEV neutralization test. The present test is useful for selective serodiagnosis.
摘要
建立了一种竞争性抑制酶联免疫吸附测定法（ELISA），以单克隆抗体为指示剂检测猪血清中针对传染性胃肠炎病毒（TGEV）纤突蛋白的非中和抗体。结果表明，与TGEV相关的猪呼吸道冠状病毒（PRCV）的野毒株不诱导产生这种抗体，而TGEV的Miller株和野毒株则能诱导产生。竞争性抑制ELISA的灵敏度似乎与病毒中和（VN）试验相似。该试验能够区分先前感染过TGEV或PRCV的猪，而这是经典的抗TGEV中和试验无法区分的。本试验对于选择性血清学诊断很有用。
相似文献
1
A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus.一种用于区分感染传染性胃肠炎病毒（TGEV）或与TGEV相关的猪呼吸道冠状病毒的猪血清抗体的竞争性抑制酶联免疫吸附测定。
Vet Microbiol. 1989 May;20(1):9-19. doi: 10.1016/0378-1135(89)90003-5.
2
3
Competition ELISA, using monoclonal antibodies to the transmissible gastroenteritis virus (TGEV) S protein, for serologic differentiation of pigs infected with TGEV or porcine respiratory coronavirus.竞争酶联免疫吸附测定法，使用针对传染性胃肠炎病毒（TGEV）S蛋白的单克隆抗体，用于对感染TGEV或猪呼吸道冠状病毒的猪进行血清学鉴别。
Am J Vet Res. 1993 Feb;54(2):254-9.
4
Serological studies of transmissible gastroenteritis in Great Britain, using a competitive ELISA.英国使用竞争性酶联免疫吸附测定法对传染性胃肠炎进行的血清学研究。
Vet Rec. 1991 May 25;128(21):500-3. doi: 10.1136/vr.128.21.500.
5
Infection with porcine respiratory coronavirus does not fully protect pigs against intestinal transmissible gastroenteritis virus.感染猪呼吸道冠状病毒不能使猪完全抵御肠道传染性胃肠炎病毒。
Vet Rec. 1989 Jul 15;125(3):58-60. doi: 10.1136/vr.125.3.58.
6
Evaluation of the baculovirus-expressed S glycoprotein of transmissible gastroenteritis virus (TGEV) as antigen in a competition ELISA to differentiate porcine respiratory coronavirus from TGEV antibodies in pigs.评价杆状病毒表达的传染性胃肠炎病毒（TGEV）S糖蛋白作为抗原在竞争ELISA中区分猪呼吸道冠状病毒抗体与猪TGEV抗体的能力。
J Vet Diagn Invest. 1999 May;11(3):205-14. doi: 10.1177/104063879901100301.
7
Field validation of a commercial blocking ELISA to differentiate antibody to transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus and to identify TGEV-infected swine herds.一种用于区分抗传染性胃肠炎病毒（TGEV）抗体和猪呼吸道冠状病毒抗体以及鉴定TGEV感染猪群的商业化阻断ELISA的现场验证
J Vet Diagn Invest. 2002 Mar;14(2):97-105. doi: 10.1177/104063870201400202.
8
Induction of milk IgA antibodies by porcine respiratory coronavirus infection.猪呼吸道冠状病毒感染诱导乳汁IgA抗体产生
Adv Exp Med Biol. 1990;276:421-8. doi: 10.1007/978-1-4684-5823-7_58.
9
Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera.用于检测猪血清中传染性胃肠炎病毒抗体的酶联免疫吸附测定
Am J Vet Res. 1984 Aug;45(8):1654-7.
引用本文的文献
1
Transmissible Gastroenteritis Virus (TGEV) and Porcine Respiratory Coronavirus (PRCV): Epidemiology and Molecular Characteristics-An Updated Overview.传染性胃肠炎病毒（TGEV）和猪呼吸道冠状病毒（PRCV）：流行病学与分子特征——最新综述
Viruses. 2025 Mar 28;17(4):493. doi: 10.3390/v17040493.
2
Establishment of a pseudovirus neutralization assay for TGEV.猪传染性胃肠炎病毒假病毒中和试验的建立。
Front Immunol. 2025 Apr 10;16:1558604. doi: 10.3389/fimmu.2025.1558604. eCollection 2025.
3
Rapid and visual detection of transmissible gastroenteritis virus using a CRISPR/Cas12a system combined with loop-mediated isothermal amplification.使用CRISPR/Cas12a系统结合环介导等温扩增技术快速可视化检测传染性胃肠炎病毒
BMC Vet Res. 2025 Apr 2;21(1):234. doi: 10.1186/s12917-025-04711-1.
4
First report of transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus (PRCV) in pigs from Poland.波兰首次报告猪传染性胃肠炎病毒（TGEV）和猪呼吸冠状病毒（PRCV）。
BMC Vet Res. 2024 Nov 18;20(1):517. doi: 10.1186/s12917-024-04364-6.
5
Development of a multiplex RT-PCR method for the detection of four porcine enteric coronaviruses.一种用于检测四种猪肠道冠状病毒的多重逆转录聚合酶链反应方法的开发。
Front Vet Sci. 2022 Nov 8;9:1033864. doi: 10.3389/fvets.2022.1033864. eCollection 2022.
6
Development and Primary Application of an Indirect ELISA Based on Rep Protein to Analyze Antibodies against Porcine Cocirvirus-like Virus (PCLV).基于 Rep 蛋白的间接 ELISA 的建立及其在猪圆环病毒样病毒（PCLV）抗体分析中的初步应用。
Viruses. 2022 Jun 27;14(7):1398. doi: 10.3390/v14071398.
7
Evolution, Interspecies Transmission, and Zoonotic Significance of Animal Coronaviruses.动物冠状病毒的进化、跨物种传播及人畜共患病意义
Front Vet Sci. 2021 Oct 18;8:719834. doi: 10.3389/fvets.2021.719834. eCollection 2021.
8
Porcine Coronaviruses: Overview of the State of the Art.猪冠状病毒：研究现状概述。
Virol Sin. 2021 Oct;36(5):833-851. doi: 10.1007/s12250-021-00364-0. Epub 2021 Mar 15.
9
Role of Porcine Aminopeptidase N and Sialic Acids in Porcine Coronavirus Infections in Primary Porcine Enterocytes.猪氨基肽酶 N 和唾液酸在猪冠状病毒感染原代猪肠上皮细胞中的作用。
Viruses. 2020 Apr 5;12(4):402. doi: 10.3390/v12040402.
10
Evaluation of the Serologic Cross-Reactivity between Transmissible Gastroenteritis Coronavirus and Porcine Respiratory Coronavirus Using Commercial Blocking Enzyme-Linked Immunosorbent Assay Kits.采用商品化的阻断酶联免疫吸附试剂盒评估传染性胃肠炎冠状病毒与猪呼吸冠状病毒之间的血清学交叉反应性。
mSphere. 2019 Mar 13;4(2):e00017-19. doi: 10.1128/mSphere.00017-19.
本文引用的文献
1
Vaccination of pregnant sows against transmissible gastroenteritis with two attenuated virus strains and different inoculation routes.给怀孕母猪接种两种减毒病毒株和不同接种途径的传染性胃肠炎疫苗。
Vet Q. 1980 Oct;2(4):211-9. doi: 10.1080/01652176.1980.9693783.
2
A transmissible gastroenteritis in pigs.猪的一种传染性胃肠炎。
J Am Vet Med Assoc. 1946 Apr;108:257-9.
3
On the pathogenesis of transmissible gastroenteritis of swine.论猪传染性胃肠炎的发病机制。
J Am Vet Med Assoc. 1972 Feb 15;160(4):534-40.
4
New porcine coronavirus?新型猪冠状病毒？
Vet Rec. 1986 Sep 13;119(11):282-3. doi: 10.1136/vr.119.11.282.
5
Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis.一种与猪传染性胃肠炎相关的猪呼吸道、非肠道冠状病毒的分离
Vet Q. 1986 Jul;8(3):257-61. doi: 10.1080/01652176.1986.9694050.
6
Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus.猪传染性胃肠炎病毒与一种相关的猪呼吸道冠状病毒之间的抗原分化
J Gen Virol. 1988 Jul;69 ( Pt 7):1725-30. doi: 10.1099/0022-1317-69-7-1725.
7
Differentiation of porcine coronavirus from transmissible gastroenteritis virus.猪冠状病毒与传染性胃肠炎病毒的鉴别
Vet Rec. 1988 Jan 23;122(4):86-7. doi: 10.1136/vr.122.4.86.
8
Antigenic variation of porcine transmissible gastroenteritis virus detected by monoclonal antibodies.利用单克隆抗体检测猪传染性胃肠炎病毒的抗原变异
Vet Microbiol. 1987 Jun;14(2):115-24. doi: 10.1016/0378-1135(87)90003-4.
9
Critical epitopes in transmissible gastroenteritis virus neutralization.传染性胃肠炎病毒中和作用中的关键表位
J Virol. 1986 Oct;60(1):131-9. doi: 10.1128/JVI.60.1.131-139.1986.
10
Antigenic structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins.传染性胃肠炎病毒的抗原结构。I. 针对病毒粒子蛋白的单克隆抗体的特性。
J Gen Virol. 1986 Jan;67 ( Pt 1):119-30. doi: 10.1099/0022-1317-67-1-119.
Zotero 插件