Mitochondrial oxidative stress and dysfunction induced by single- and multiwall carbon nanotubes: A comparative study.

With the ever-increasing use of carbon nanotubes (CNTs) in health-related and engineering applications, the hazardous risks of this material have become a major concern. It is well known that CNTs accumulate with cytotoxic and genotoxic levels within vital organs. It has also been shown that treating cell cultures with CNTs resulted in cell-cycle arrest and increased apoptosis/necrosis. The goal of this pilot study is to perform a comprehensive comparative study on the toxicity of single-wall (SW) and multiwall (MW) CNTs in rat skin cells. Our results confirm a dose-dependent toxicity of SWCNTs and MWCNTs due to the loss of mitochondrial activity, increase in mitochondrial reactive oxygen species (ROS) formation, and mitochondrial membrane potential collapse before mitochondrial swelling. Moreover, disturbance in the oxidative phosphorylation is observed by a decrease in ATP level. These events induced the release of cytochrome c via outer membrane rupture or MPT pore opening and subsequently programmed cell death of all doses compared to control group. Our results demonstrate that although MWCNTs can be very toxic, SWCNTs cause more mitochondrial damage to the cells. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2047-2055, 2017.