Sharbatdaran Majid, Abbaszadeh Hamid, Siadati Sepideh, Ranaee Mohammad, Hajian-Tilaki Karimollah, Rajabi-Moghaddam Mahdieh
Department of Pathology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Babol University of Medical Sciences, Babol, Iran.
Diagn Cytopathol. 2017 Jun;45(6):513-519. doi: 10.1002/dc.23706. Epub 2017 Mar 10.
Smoking is the most important etiologic factor of oral cancer. Exfoliative cytology is the best method for early detection of oral cancer. Toluidine blue staining is used for detection of oral premalignant and malignant lesions. The aim of this study was to enhance the accuracy of oral exfoliative cytology in evaluating dysplastic features using toluidine blue staining.
This clinical trials study was performed on 60 male smokers and nonsmokers without clinically oral lesion. Oral exfoliative cytological smears were prepared before and after application of toluidine blue and stained with Papanicolaou and evaluated under light microscope. Cytological features such as cellular clumping nuclear-to-cytoplasmic ratio, cellular and nuclear pleomorphism, micronuclei, binucleation, presence of bacterial colonies, and keratin flakes were assessed and compared before and after application of toluidine blue.
Results showed that cellular clumping and micronuclei were significantly decreased after application of toluidine blue and conversely cellular and nuclear pleomorphisms were significantly increased. Frequency of micronuclei and binucleation were greater in smokers than nonsmokers which were insignificant. Cellular and nuclear pleomorphisms were significantly higher in smokers than nonsmokers after application of toluidine blue.
Toluidine blue improved cellular, nuclear, and structural features of oral cytological smears and filtered false-positive or false-negative results. Thus, application of toluidine blue in combination with oral exfoliative cytology for early detection of oral cancer is recommended. Diagn. Cytopathol. 2017;45:513-519. © 2017 Wiley Periodicals, Inc.
吸烟是口腔癌最重要的病因。脱落细胞学检查是早期发现口腔癌的最佳方法。甲苯胺蓝染色用于检测口腔癌前病变和恶性病变。本研究的目的是提高口腔脱落细胞学检查在使用甲苯胺蓝染色评估发育异常特征方面的准确性。
本临床试验研究对60名无临床口腔病变的男性吸烟者和非吸烟者进行。在应用甲苯胺蓝前后制备口腔脱落细胞学涂片，用巴氏染色法染色，并在光学显微镜下评估。评估并比较应用甲苯胺蓝前后的细胞学特征，如细胞聚集、核质比、细胞和核多形性、微核、双核、细菌菌落的存在以及角质鳞片。
结果显示，应用甲苯胺蓝后细胞聚集和微核显著减少，相反，细胞和核多形性显著增加。吸烟者的微核和双核频率高于非吸烟者，但差异不显著。应用甲苯胺蓝后，吸烟者的细胞和核多形性显著高于非吸烟者。
甲苯胺蓝改善了口腔细胞学涂片的细胞、核和结构特征，并过滤了假阳性或假阴性结果。因此，建议将甲苯胺蓝与口腔脱落细胞学检查联合应用于口腔癌的早期检测。诊断细胞病理学。2017；45：513 - 519。©2017威利期刊公司。
