通过偶联核苷酸转移酶测定核苷二磷酸激酶活性。
Assay of nucleoside-diphosphate kinase activity by the coupled nucleotidyltransferase.
作者信息
Sato N L
机构信息
Facilities for Comparative Medicine & Animal Experimentation, Niigata University, School of Medicine, Japan.
出版信息
Anal Biochem. 1987 Nov 1;166(2):284-6. doi: 10.1016/0003-2697(87)90576-8.
PMID:2829652
Abstract
A method coupled with Escherichia coli RNA polymerase is described for assaying nucleoside-diphosphate kinase activity. The principle of the procedure is indirect but allows processing of a large number of samples by employing filter-paper disk techniques.
摘要
描述了一种与大肠杆菌RNA聚合酶相结合的方法，用于测定核苷二磷酸激酶活性。该方法的原理是间接的，但通过采用滤纸圆盘技术，可以处理大量样品。
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