Kugo H, Zaima N, Tanaka H, Urano T, Unno N, Moriyama T
a Department of Applied Biological Chemistry, Graduate School of Agricultural Science , Kindai University , Nara.
b Department of Medical Physiology , Hamamatsu University School of Medicine , Shizuoka.
Biotech Histochem. 2017;92(2):141-148. doi: 10.1080/10520295.2017.1287428. Epub 2017 Feb 28.
Abdominal aortic aneurysm (AAA) is the progressive dilation of the abdominal aorta. Nicotine is reported to be associated with the development and rupture of AAA, but the pathological effects of nicotine on normal rat aorta have not been determined. We investigated pathological changes in the aortic wall of rats caused by the administration of nicotine. Nicotine administration weakened the vascular wall, increased gelatinolytic activity and promoted the destruction of elastin and collagen in the rat abdominal aorta. There were no differences in the areas positive for matrix metalloproteinase (MMP)-2 and MMP-9 between the control and nicotine treated groups. The areas positive for MMP-12 in the nicotine group were significantly greater than for the control group. Gelatinolytic activity in the aortic wall was increased significantly in the nicotine group. Our findings suggest that MMP-12 is sensitive to nicotine exposure in rats.
腹主动脉瘤（AAA）是腹主动脉的进行性扩张。据报道，尼古丁与AAA的发生和破裂有关，但尼古丁对正常大鼠主动脉的病理影响尚未确定。我们研究了尼古丁给药对大鼠主动脉壁的病理变化。尼古丁给药削弱了血管壁，增加了明胶酶活性，并促进了大鼠腹主动脉中弹性蛋白和胶原蛋白的破坏。对照组和尼古丁处理组之间基质金属蛋白酶（MMP）-2和MMP-9阳性区域没有差异。尼古丁组中MMP-12阳性区域明显大于对照组。尼古丁组主动脉壁中的明胶酶活性显著增加。我们的研究结果表明，MMP-12对大鼠暴露于尼古丁敏感。
