Willard M D, Refsal K, Thacker E
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing 48824-1314.
Am J Vet Res. 1987 Dec;48(12):1713-8.
Plasma aldosterone concentrations were measured in response to adrenocorticotropic hormone (ACTH) gel administration in clinically normal dogs, in dogs with hypoadrenocorticism, and in dogs (with electrolyte abnormalities) that did not have hypoadrenocorticism. Baseline plasma aldosterone concentrations were determined from specimens obtained every 10 minutes for 3 hours from 2 dogs and every 30 minutes for 7.5 hours from 2 other dogs. During the evaluation period, plasma aldosterone concentrations varied by at least 50% in each dog. A randomized crossover design was used to compare changes in plasma aldosterone concentrations after administration of ACTH gel and physiologic NaCl solution. Dogs had significantly (P = 0.002) higher plasma aldosterone concentrations after administration of ACTH gel than after administration of NaCl solution. Plasma cortisol concentrations increased as expected after ACTH gel administration. Analysis of cortisol and aldosterone concentrations in the same specimens obtained at 7 sample collection times did not reveal significant linear correlation, and scatterplots did not indicate a nonlinear association. In addition, plasma aldosterone concentrations were determined in response to ACTH administration alone and to ACTH combined with a high dose of dexamethasone (0.1 mg/kg, IV). The plasma aldosterone response to ACTH alone was not significantly different from the response to ACTH combined with dexamethasone. For both tests, plasma aldosterone concentrations at 60 and 120 minutes after ACTH administration were significantly (P less than 0.0005 and P = 0.0001, respectively, increased, compared with base-line values. Six dogs with adrenocortical hypofunction, as determined by plasma cortisol concentrations before and after ACTH administration, had plasma aldosterone concentrations that were diminished or did not increase after ACTH administration.(ABSTRACT TRUNCATED AT 250 WORDS)
在临床正常犬、肾上腺皮质功能减退犬以及无肾上腺皮质功能减退但有电解质异常的犬中，测量了给予促肾上腺皮质激素（ACTH）凝胶后血浆醛固酮浓度。从2只犬每10分钟采集一次标本，共采集3小时，从另外2只犬每30分钟采集一次标本，共采集7.5小时，以此来确定基线血浆醛固酮浓度。在评估期间，每只犬的血浆醛固酮浓度变化至少达50%。采用随机交叉设计比较给予ACTH凝胶和生理氯化钠溶液后血浆醛固酮浓度的变化。给予ACTH凝胶后犬的血浆醛固酮浓度显著高于给予氯化钠溶液后（P = 0.002）。给予ACTH凝胶后血浆皮质醇浓度如预期升高。对在7个采样时间点采集的相同标本中的皮质醇和醛固酮浓度进行分析，未发现显著的线性相关性，散点图也未显示非线性关联。此外，还分别测量了单独给予ACTH以及ACTH与高剂量地塞米松（0.1 mg/kg，静脉注射）联合应用后血浆醛固酮浓度。单独给予ACTH时血浆醛固酮反应与ACTH联合地塞米松时的反应无显著差异。对于这两种试验，给予ACTH后60分钟和120分钟时的血浆醛固酮浓度与基线值相比均显著升高（分别为P < 0.0005和P = 0.0001）。根据给予ACTH前后血浆皮质醇浓度确定的6只肾上腺皮质功能减退犬，给予ACTH后血浆醛固酮浓度降低或未升高。（摘要截短至250字）
