Division of Cardiology, Emory Clinical Cardiovascular Research Institute, Emory University School of Medicine, Atlanta, Georgia, USA.
Lexington VA Medical Center and Saha Cardiovascular Research Center, University of Kentucky, Lexington, Kentucky, USA.
Stem Cells Transl Med. 2017 Mar;6(3):731-735. doi: 10.5966/sctm.2016-0059. Epub 2016 Oct 14.
Bone marrow-derived progenitor cells are mobilized into the peripheral blood after acute myocardial injury and in chronic ischemic heart disease. However, the mechanisms responsible for this mobilization are poorly understood. We examined the relationship between plasma levels of bioactive lipids and number of circulating progenitor cells (CPCs) in patients (N = 437) undergoing elective or emergent cardiac catheterization. Plasma levels of sphingosine-1 phosphate (S1P) and ceramide-1 phosphate (C1P) were quantified using mass spectrometry. CPCs were assessed using flow cytometry. S1P levels correlated with the numbers of CD34+, CD34+/CD133+, and CD34+/CXCR4+ CPCs even after adjustment for potential confounding factors. However, no significant correlation was observed between C1P levels and CPC count. Plasma levels of S1P correlated with the number of CPCs in patients with coronary artery disease, suggesting an important mechanistic role for S1P in stem cell mobilization. The therapeutic effects of adjunctive S1P therapy to mobilize endogenous stem cells need to be investigated. Stem Cells Translational Medicine 2017;6:731-735.
骨髓源性祖细胞在急性心肌损伤和慢性缺血性心脏病后动员到外周血中。然而，负责这种动员的机制尚不清楚。我们检查了在接受选择性或紧急心导管检查的患者（N = 437）中，血浆中生物活性脂质水平与循环祖细胞（CPC）数量之间的关系。使用质谱法定量测定鞘氨醇-1 磷酸（S1P）和神经酰胺-1 磷酸（C1P）的水平。使用流式细胞术评估 CPC。即使在调整了潜在的混杂因素后，S1P 水平仍与 CD34+、CD34+/CD133+和 CD34+/CXCR4+CPC 的数量相关。然而，C1P 水平与 CPC 计数之间没有显著相关性。S1P 与冠状动脉疾病患者中 CPC 数量的相关性表明 S1P 在干细胞动员中的重要作用。需要研究辅助 S1P 治疗以动员内源性干细胞的治疗效果。《干细胞转化医学》2017；6:731-735。
