Xie Pingyao, Cui Lili, Shan Yuan, Kang Wen-Yi
Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng 475004, China.
Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.
Biomed Res Int. 2017;2017:9475074. doi: 10.1155/2017/9475074. Epub 2017 Feb 16.
The compounds of Radix Paeoniae Rubra (RPR) were isolated and identified by bioassay-guided method, and antithrombotic effects and mechanism were investigated by the acute blood stasis rat model. The RPR extract was evaluated by APTT, TT, PT, and FIB assays in vitro. Results indicated that RPR extract exhibited the anticoagulant activity. In order to find active compounds, six compounds were isolated and identified, and four compounds, paeoniflorin (Pae), pentagalloylglucose (Pen), albiflorin (Ali), and protocatechuic acid (Pro), exhibited the anticoagulant activity in vitro. Therefore, the antithrombosis effects of RPR extract and four active compounds were investigated in vivo by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of TXB, 6-Keto-PGF , eNOS, and ET-1 were detected. Results suggested that RPR extract and four active compounds had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect.
采用生物活性追踪法对赤芍（RPR）的化合物进行分离鉴定，并通过急性血瘀大鼠模型研究其抗血栓作用及机制。体外采用活化部分凝血活酶时间（APTT）、凝血酶时间（TT）、血浆凝血酶原时间（PT）及纤维蛋白原（FIB）测定法对RPR提取物进行评价。结果表明，RPR提取物具有抗凝活性。为寻找活性成分，分离鉴定了6个化合物，其中芍药苷（Pae）、五倍子酰葡萄糖（Pen）、 albiflorin（Ali）和原儿茶酸（Pro）4个化合物具有体外抗凝活性。因此，通过检测全血黏度（WBV）、血浆黏度（PV）、APTT、PT、TT及FIB，在体内研究了RPR提取物及4个活性化合物的抗血栓作用。同时，检测血栓素B2（TXB）、6-酮-前列腺素F1α（6-Keto-PGF）、内皮型一氧化氮合酶（eNOS）及内皮素-1（ET-1）水平。结果提示，RPR提取物及4个活性化合物对血栓形成具有抑制作用，其抗血栓作用与调节血管内皮活性物质、促进血流及抗凝作用有关。
