来自紫藤的一种新型杆状DNA病毒的鉴定
Characterization of a new badnavirus from Wisteria sinensis.
作者信息
Li Yongqiang, Deng Congliang, Qiao Yan, Zhao Xiaoli, Zhou Qi
机构信息
College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China.
出版信息
Arch Virol. 2017 Jul;162(7):2125-2129. doi: 10.1007/s00705-017-3322-4. Epub 2017 Mar 15.
The complete genome sequence of a previously undescribed badnavirus isolated from a wisteria plant exhibiting mosaic and crinkle symptoms in Beijing, China, was determined. The circular double-stranded DNA genome of this virus was 7362 bp in size with four open reading frames (ORFs 1 to 4) on the plus strand. Sequence analysis showed that this virus shared the highest (69%) nucleotide (nt) sequence identity with pagoda yellow mosaic associated virus (PYMAV). In the RT-RNase H region of the ORF-3 encoded polyprotein, this virus shared 74% nt sequence identity with PYMAV. Phylogenetic analysis provided further evidence that the virus identified in this study is a member of a new species in the genus Badnavirus. The name wisteria badnavirus 1 (WBV1) is proposed for this new virus.
测定了从中国北京一株表现出花叶和皱缩症状的紫藤植物中分离出的一种此前未描述过的杆状DNA病毒的全基因组序列。该病毒的环状双链DNA基因组大小为7362 bp，正链上有四个开放阅读框（ORF 1至4）。序列分析表明，该病毒与宝塔黄花叶相关病毒（PYMAV）的核苷酸（nt）序列同一性最高（69%）。在ORF-3编码的多蛋白的RT-RNase H区域，该病毒与PYMAV的nt序列同一性为74%。系统发育分析进一步证明，本研究中鉴定的病毒是杆状DNA病毒属一个新物种的成员。建议将这种新病毒命名为紫藤杆状DNA病毒1（WBV1）。
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