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首次鉴定和分子特征分析一种侵染茶花的新马铃薯 Y 病毒属病毒。

First identification and molecular characterization of a new badnavirus infecting camellia.

机构信息

USDA-ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.

College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

出版信息

Arch Virol. 2020 Sep;165(9):2115-2118. doi: 10.1007/s00705-020-04698-0. Epub 2020 Jun 19.

Abstract

A new badnavirus was identified in an ornamental camellia tree with yellow mottle symptom. The complete circular double-stranded DNA genome of this virus was found to consist of 8,203 bp. Its genome organization is typical of badnaviruses, containing three open reading frames (ORFs). ORFs 1 and 2 encode putative proteins with unknown functions. ORF3 encodes a large polyprotein that contains almost all of the conserved domains of badnaviruses. The virus shares 55-62% nucleotide sequence identities with other badnaviruses in the RT+RNase H region. Phylogenetic analyses placed it in group I of the genus Badnavirus. Therefore, this virus, which is tentatively named "camellia Lemon Glow virus", should represent a new species of the genus Badnavirus. This virus was found to be present in approximately a quarter of camellia trees tested.

摘要

一种新的伴生病毒在一棵具有黄斑斑驳症状的观赏茶花树上被发现。该病毒的完整圆形双链 DNA 基因组由 8203 个碱基对组成。其基因组结构是典型的伴生病毒,包含三个开放阅读框(ORFs)。ORF1 和 ORF2 编码具有未知功能的假定蛋白。ORF3 编码一个大的多蛋白,其中包含几乎所有伴生病毒的保守结构域。该病毒在 RT+RNase H 区域与其他伴生病毒的核苷酸序列同一性为 55-62%。系统发育分析将其置于伴生病毒属的 I 组。因此,这种病毒,暂定名为“茶花柠檬光病毒”,应该代表伴生病毒属的一个新种。在大约四分之一的受测茶花树上发现了这种病毒。

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