Umar M, Amer M A, Al-Saleh M A, Al-Shahwan I M, Shakeel M T, Zakri A M, Katis N I
Plant Protection Department, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi Arabia.
Viruses and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
Arch Virol. 2017 Jul;162(7):2067-2072. doi: 10.1007/s00705-017-3319-z. Epub 2017 Mar 16.
During 2014 and 2015, 97 lettuce plants that showed big-vein-disease-like symptoms and seven weed plants were collected from the Riyadh region. DAS-ELISA revealed that 25% and 9% of the lettuce plants were singly infected with LBVaV and MiLBVV, respectively, whereas 63% had a mixed infection with both viruses. The results were confirmed by multiplex reverse transcription polymerase chain reaction using primers specific for LBVaV and MiLBVV. LBVaV and MiLBVV were also detected in Sonchus oleraceus and Eruca sativa, respectively. The nucleotide sequence of LBVaV and MiLBVV Saudi isolates ranged from 94.3-100%, and their similarities to isolates with sequences in the GenBank database ranged from 93.9 to 99.6% and 93.8 to 99.3%, respectively. Olpidium sp. was present in the roots of lettuce plants with big-vein disease and it was shown to facilitate transmission of both viruses.
2014年至2015年期间，从利雅得地区采集了97株表现出类似大脉病症状的生菜植株和7株杂草植株。双抗夹心酶联免疫吸附测定（DAS-ELISA）显示，分别有25%和9%的生菜植株单独感染了生菜大脉病毒A（LBVaV）和甜瓜潜隐病毒（MiLBVV），而63%的植株同时感染了这两种病毒。使用针对LBVaV和MiLBVV的特异性引物进行多重逆转录聚合酶链反应，证实了上述结果。在苣荬菜和芝麻菜中也分别检测到了LBVaV和MiLBVV。沙特分离株的LBVaV和MiLBVV核苷酸序列相似度为94.3%-100%，它们与GenBank数据库中序列的相似性分别为93.9%至99.6%和93.8%至99.3%。在患有大脉病的生菜植株根部存在油壶菌属真菌，并且已证明它有助于两种病毒的传播。
