Haring Alexander P, Cesewski Ellen, Johnson Blake N
Department of Industrial and Systems Engineering, School of Neuroscience, Macromolecules Innovation Institute, Virginia Tech, 1145 Perry Street, Blacksburg, VA, 24061, USA.
Methods Mol Biol. 2017;1572:247-262. doi: 10.1007/978-1-4939-6911-1_17.
This chapter reviews the design, fabrication, characterization, and application of piezoelectric-excited millimeter-sized cantilever (PEMC) sensors. The sensor transduction mechanism, sensing principle, and mode of operation are discussed. Bio-recognition strategies and surface functionalization methods for detection of DNA and RNA are discussed with a focus on self-assembly-based approaches. Methods for the verification of biosensor response via secondary binding assays, reversible binding assays, and the integration of complementary transduction mechanisms are presented. Sensing applications for medical diagnostics, food safety, and environmental monitoring are provided. PEMC sensor technology provides a robust platform for the real-time, label-free detection of DNA and RNA in complex matrices over nanomolar (nM) to attomolar (aM) concentration ranges.
本章回顾了压电激发毫米级悬臂梁（PEMC）传感器的设计、制造、表征及应用。讨论了传感器的转换机制、传感原理和操作模式。重点讨论了基于自组装方法的用于检测DNA和RNA的生物识别策略及表面功能化方法。介绍了通过二次结合测定、可逆结合测定以及互补转换机制的整合来验证生物传感器响应的方法。提供了医疗诊断、食品安全和环境监测方面的传感应用。PEMC传感器技术为在纳摩尔（nM）至阿托摩尔（aM）浓度范围内实时、无标记检测复杂基质中的DNA和RNA提供了一个强大的平台。
