Zeng Lingwen, Xiao Zhuo
Institute of Environment and Safety, Wuhan Academy of Agricultural Sciences, 1 Qinglingzhangjiawan, Hongshan District, Wuhan, China.
Methods Mol Biol. 2017;1572:421-430. doi: 10.1007/978-1-4939-6911-1_27.
A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step 2, the reaction product of step 1 is detected by a lateral flow biosensor, which is a rapid and cost effective tool for nuclei acid detection. Comparing with conventional methods, it requires no complicated machines. It is suitable for the use of point of care diagnostics. Therefore, this simple, cost effective, robust, and promising LFB detection method of SNP has great potential for the detection of genetic diseases, personalized medicine, cancer related mutations, and drug-resistant mutations of infectious agents.
一种用于检测单核苷酸多态性（SNP）的侧向流动生物传感器（LFB）被引入。该检测方法由两个步骤组成：环状链置换反应和侧向流动生物传感器检测。在步骤1中，SNP位点的核苷酸被T4 DNA连接酶识别，信号通过链置换DNA聚合酶进行放大，这可以在恒温下完成。在步骤2中，步骤1的反应产物由侧向流动生物传感器检测，该传感器是一种用于核酸检测的快速且经济高效的工具。与传统方法相比，它不需要复杂的仪器。它适用于即时诊断。因此，这种简单、经济高效、稳健且有前景的SNP的LFB检测方法在遗传疾病检测、个性化医疗、癌症相关突变以及感染因子的耐药性突变检测方面具有巨大潜力。
