用于免疫测定和核酸扩增检测的微流控“袋式”芯片

Microfluidic "Pouch" Chips for Immunoassays and Nucleic Acid Amplification Tests.

作者信息

Mauk Michael G, Liu Changchun, Qiu Xianbo, Chen Dafeng, Song Jinzhao, Bau Haim H

机构信息

School of Engineering and Applied Sciences, University of Pennsylvania, 220 S 33rd Street, Philadelphia, PA, 19104, USA.

Beijing University of Chemical Technology, Beijing, China.

出版信息

Methods Mol Biol. 2017;1572:467-488. doi: 10.1007/978-1-4939-6911-1_30.

DOI:10.1007/978-1-4939-6911-1_30

PMID:28299706

Abstract

Microfluidic cassettes ("chips") for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-use test chips can be self-contained and made amenable to autonomous operation-reducing or eliminating supporting instrumentation-by incorporating laminated, pliable "pouch" and membrane structures for fluid storage, pumping, mixing, and flow control. Materials and methods for integrating flexible pouch compartments and diaphragm valves into hard plastic (e.g., acrylic and polycarbonate) microfluidic "chips" for reagent storage, fluid actuation, and flow control are described. We review several versions of these pouch chips for immunoassay and nucleic acid amplification tests, and describe related fabrication techniques. These protocols thus offer a "toolbox" of methods for storage, pumping, and flow control functions in microfluidic devices.

摘要

用于临床标本及其他样本类型处理与分析的微流控盒（“芯片”）有助于即时检测（POC）免疫测定和基于核酸的扩增检测。这些一次性测试芯片可以自成一体，并通过并入用于流体存储、泵送、混合和流量控制的层压、柔韧“袋”和膜结构，使其适合自主操作，从而减少或消除辅助仪器。本文描述了将柔性袋隔室和隔膜阀集成到硬塑料（如丙烯酸和聚碳酸酯）微流控“芯片”中以进行试剂存储、流体驱动和流量控制的材料与方法。我们回顾了这些用于免疫测定和核酸扩增检测的袋式芯片的几个版本，并描述了相关制造技术。因此，这些方案提供了一个用于微流控设备中存储、泵送和流量控制功能的方法“工具箱”。