Ayub Muhammad Adnan, Hussain Abdullah Ijaz, Hanif Muhammad Asif, Chatha Shahzad Ali Shahid, Kamal Ghulam Mustafa, Shahid Muhammad, Janneh Omar
Department of Chemistry, University of Agriculture, Faisalabad, Pakistan.
Natural Product and Synthetic Chemistry Lab., Department of Chemistry, Government College University, Faisalabad, Pakistan.
Chem Biodivers. 2017 Jun;14(6). doi: 10.1002/cbdv.201600463. Epub 2017 May 18.
The objective of present study was to evaluate the variation in phenolic profile, β-carotene, flavonoid contents, antioxidant and antimicrobial properties of Tagetes erecta and Tagetes patula (T. erecta and T. patula) through different in vitro assays. Antioxidant activity was determined through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and inhibition of linoleic acid peroxidation assays and antibacterial and antifungal activities studied using the disc diffusion and resazurin microtiter-plate assays against bacterial and fungal strains. Moreover, total phenolics (TP), total carotenoids (TC) and total flavonoids (TF) were also determined. Highest (TP 35.8 mg GAE/g) and TF (16.9 mg CE/g) contents were found in MeOH extract of T. patula. T. erecta extract showed higher TC contents (6.45 mg/g) than T. patula extract (6.32 mg/g). T. erecta exhibited the highest DPPH radical-scavenging activity (IC ) (5.73 μg/mL) and inhibition of linoleic acid peroxidation (80.1%). RP-HPLC revealed the presence of caffeic acid, sinapic acid and ferulic acid in Tagetes extracts, m-coumaric acid in T. erecta whereas chlorogenic acid in T. patula extract only. Both extracts possessed promising antimicrobial activity compared to the ciprofloxacin and flumequine (+ve controls) against Bacillus subtilis and Alternaria alternate. Both extract were rich source of polyphenols exhibiting excellent biological activities.
本研究的目的是通过不同的体外试验评估万寿菊和孔雀草（万寿菊和孔雀草）的酚类成分、β-胡萝卜素、类黄酮含量、抗氧化和抗菌性能的变化。通过2,2-二苯基-1-苦基肼（DPPH）自由基清除和亚油酸过氧化抑制试验测定抗氧化活性，并使用纸片扩散法和刃天青微量滴定板试验研究对细菌和真菌菌株的抗菌和抗真菌活性。此外，还测定了总酚（TP）、总类胡萝卜素（TC）和总黄酮（TF）。孔雀草的甲醇提取物中TP含量最高（35.8 mg GAE/g），TF含量最高（16.9 mg CE/g）。万寿菊提取物的TC含量（6.45 mg/g）高于孔雀草提取物（6.32 mg/g）。万寿菊表现出最高的DPPH自由基清除活性（IC）（5.73 μg/mL）和亚油酸过氧化抑制率（80.1%）。RP-HPLC分析表明，万寿菊提取物中存在咖啡酸、芥子酸和阿魏酸，万寿菊中存在间香豆酸，而孔雀草提取物中仅存在绿原酸。与环丙沙星和氟甲喹（阳性对照）相比，两种提取物对枯草芽孢杆菌和链格孢均具有良好的抗菌活性。两种提取物都是富含多酚的来源，具有优异的生物活性。
