12p12染色体改变的急性淋巴细胞白血病细胞中的Ki-ras-2

Ki-ras-2 in acute lymphoblastic leukemia cells with chromosome change at 12p12.

作者信息

Ohyashiki K, Ohyashiki J H, Kinniburgh A J, Toyama K, Ito M, Minowada J, Sandberg A A

机构信息

Department of Genetics and Endocrinology, Roswell Park Memorial Institute, Buffalo, NY.

出版信息

Cancer Genet Cytogenet. 1988 Feb;30(2):239-44. doi: 10.1016/0165-4608(88)90190-2.

DOI:10.1016/0165-4608(88)90190-2

PMID:2830012

Abstract

Cytogenetic and molecular investigations were performed on a pre-B-lymphoblastic acute leukemic cell line (NALM-6). The NALM-6 cells contained a del(5)(q32) and an ins(12)(p12;?), chromosomal material of unknown origin being inserted between subbands 12p12.1 and 12p12.2. Chromosomal in situ hybridization using a 3.0-kb c-Ki-ras-2 probe showed a significant accumulation of grains on the proximal portion of the inserted chromosomal material (12p12.1), as well as on the normal chromosome #12 with a peak at 12p11p12. The signal intensity obtained after hybridization of the c-Ki-ras-2 specific probe to the NALM-6 cells DNA is comparable with the intensity of the signal after hybridization of the same probe with the control cell line (MC/B) DNA. The findings indicate that the c-Ki-ras-2 gene is neither amplified nor transposed in the NALM-6 cells.

摘要

对一株前B淋巴细胞性急性白血病细胞系（NALM-6）进行了细胞遗传学和分子研究。NALM-6细胞含有一条del(5)(q32)和一条ins(12)(p12;?)，未知来源的染色体物质插入在12p12.1和12p12.2亚带之间。使用3.0-kb c-Ki-ras-2探针进行染色体原位杂交显示，在插入的染色体物质近端部分（12p12.1）以及正常的12号染色体上，12p11p12处出现明显的颗粒聚集。将c-Ki-ras-2特异性探针与NALM-6细胞DNA杂交后获得的信号强度，与该探针与对照细胞系（MC/B）DNA杂交后的信号强度相当。这些发现表明，c-Ki-ras-2基因在NALM-6细胞中既未扩增也未转位。