Fukumoto M, Suzuki A, Inazawa J, Yoshimura T, Arao S, Takahashi T, Nomura H, Hiai H
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
Somat Cell Mol Genet. 1993 Jan;19(1):21-8. doi: 10.1007/BF01233951.
Gene amplification is a major mechanism through which oncogenes and genes responsible for drug resistance are overexpressed in neoplastic cells, and several models for structure of amplified units (amplicons) are postulated. In order to identify consistent changes associated with oncogene amplification, we analyzed chromosomal location and physical distance of amplicons of two independent human cell lines that have coamplified c-myc and Ki-ras oncogenes. In one cell line, KHC287, amplified c-myc genes were localized in two chromosomes and Ki-ras in three chromosomes. One marker chromosome was almost entirely encompassed by both amplified genes. In the other cell line, Lu-65, both of the amplified genes shared the same locus, on chromosome 12q+. The two genes, however, are more than 1500 kb apart in both cell lines. The above findings indicate that two different amplified genes became associated on one chromosome in two independent cell lines. This suggests that a common mechanism is associated with chromosomal rearrangements affecting different amplified genes.
基因扩增是肿瘤细胞中癌基因和耐药相关基因过度表达的主要机制，并且推测了几种扩增单元（扩增子）的结构模型。为了确定与癌基因扩增相关的一致性变化，我们分析了两个独立的共扩增c-myc和Ki-ras癌基因的人类细胞系中扩增子的染色体定位和物理距离。在一个细胞系KHC287中，扩增的c-myc基因定位于两条染色体，Ki-ras定位于三条染色体。一条标记染色体几乎完全被两个扩增基因所包围。在另一个细胞系Lu-65中，两个扩增基因位于12q +染色体的同一位置。然而，在两个细胞系中，这两个基因相距超过1500 kb。上述发现表明，在两个独立的细胞系中，两个不同的扩增基因在一条染色体上发生了关联。这表明一种共同机制与影响不同扩增基因的染色体重排有关。
