SRSMC UMR 7565 CNRS-Université de Lorraine, Bvd des Aiguillettes, BP 70239, F-54506 Vandoeuvre-lès-Nancy, France.
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, University of Alcalá , E-28871 Alcala de Henares, Spain.
Langmuir. 2017 Apr 4;33(13):3333-3340. doi: 10.1021/acs.langmuir.7b00134. Epub 2017 Mar 27.
β-Galactosidase (β-Gal) is one of the most important enzymes used in milk processing for improving their nutritional quality and digestibility. Herein, β-Gal has been entrapped into a meso-macroporous material (average pore size 9 and 200 nm, respectively) prepared by a sol-gel method from a silica precursor and a dispersion of solid lipid nanoparticles in a micelle phase. The physisorption of the enzyme depends on the concentration of the feed solution and on the pore size of the support. The enzyme is preferentially adsorbed either in mesopores or in macropores, depending on its initial concentration. Moreover, this selective adsorption, arising from the oligomeric complexation of the enzyme (monomer/dimer/tetramer), has an effect on the catalytic activity of the material. Indeed, the enzyme encapsulated in macropores is more active than the enzyme immobilized in mesopores. Designed materials containing β-Gal are of particular interest for food applications and potentially extended to bioconversion, bioremediation, or biosensing when coupling the designed support with other enzymes.
β-半乳糖苷酶（β-Gal）是乳制品加工中最重要的酶之一，用于改善其营养价值和消化率。在此，β-Gal 已被包埋在由硅前体和固体脂质纳米粒子在胶束相中的分散体制备的中孔-大孔材料中。酶的物理吸附取决于进料溶液的浓度和载体的孔径。根据其初始浓度，酶优先吸附在介孔或大孔中。此外，这种选择性吸附源于酶的低聚复合（单体/二聚体/四聚体），对材料的催化活性有影响。实际上，包埋在大孔中的酶比固定在介孔中的酶更具活性。含有β-Gal 的设计材料对于食品应用特别有意义，并有可能在与其他酶偶联时扩展到生物转化、生物修复或生物传感。
