Vireque Alessandra A, Tata Alessandra, Belaz Katia Roberta A, Grázia João Gabriel V, Santos Fábio N, Arnold Daniel R, Basso Andrea C, Eberlin Marcos N, Silva-de-Sá Marcos Felipe, Ferriani Rui A, Sá Rosa-E-Silva Ana Carolina J
a Department of Obstetrics and Gynecology, School of Medicine of Ribeirão Preto , University of São Paulo , Ribeirão Preto , SP , Brazil.
b ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry , University of Campinas , Campinas , SP , Brazil.
Syst Biol Reprod Med. 2017 Apr;63(2):86-99. doi: 10.1080/19396368.2017.1289279. Epub 2017 Feb 28.
The influence of cumulus cells (CC) on the lipid profile of bovine oocytes matured in two different lipid sources was investigated. Cumulus-oocyte complexes (COC) or denuded oocytes (DO) were matured in tissue culture medium (TCM) supplemented with fetal bovine serum (FBS) or serum substitute supplement (SSS). Lipid profiles of TCM, serum supplements, immature CC and oocyte (IO), and in vitro-matured oocytes from COC and DO were then analyzed by matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) and submitted to partial least squares-discriminant analysis (PLS-DA). The developmental competence of such oocytes was also assessed. Differences in lipid composition were observed between two types of sera and distinctly influenced the lipid profile of CC. As revealed by PLS-DA, the abundance of specific ions corresponding to triacylglycerols (TAG) or phospholipids (PL) were higher in COC compared to DO both supplemented with FBS or SSS and to some extent affected the subsequent DO in vitro embryo development. DO exposed to SSS had however a marked diminished ability to develop to the blastocyst stage. These results indicate a modulation by CC of the oocyte TAG and PL profiles associated with a specific cell response to the serum supplement used for in vitro maturation.
研究了卵丘细胞（CC）对在两种不同脂质来源中成熟的牛卵母细胞脂质谱的影响。卵丘-卵母细胞复合体（COC）或裸卵（DO）在补充有胎牛血清（FBS）或血清替代补充剂（SSS）的组织培养基（TCM）中成熟。然后通过基质辅助激光解吸电离质谱（MALDI-MS）分析TCM、血清补充剂、未成熟CC和卵母细胞（IO）以及来自COC和DO的体外成熟卵母细胞的脂质谱，并进行偏最小二乘判别分析（PLS-DA）。还评估了此类卵母细胞的发育能力。观察到两种血清之间脂质组成存在差异，且明显影响了CC的脂质谱。如PLS-DA所示，与补充有FBS或SSS的DO相比，COC中对应于三酰甘油（TAG）或磷脂（PL）的特定离子丰度更高，并且在一定程度上影响了随后DO的体外胚胎发育。然而，暴露于SSS的DO发育到囊胚阶段的能力明显下降。这些结果表明CC对卵母细胞TAG和PL谱有调节作用，这与对用于体外成熟的血清补充剂的特定细胞反应有关。
