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miR-202-5p通过靶向ACSL3抑制鹅等级颗粒细胞的脂质代谢和类固醇生成。

miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3.

作者信息

Ran Mingxia, Hu Shenqiang, Ouyang Qingyuan, Xie Hengli, Zhang Xi, Lin Yueyue, Li Xuejian, Hu Jiwei, Li Liang, He Hua, Liu Hehe, Wang Jiwen

机构信息

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Animals (Basel). 2023 Jan 17;13(3):325. doi: 10.3390/ani13030325.

DOI:10.3390/ani13030325
PMID:36766213
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9913746/
Abstract

miRNAs are critical for steroidogenesis in granulosa cells (GCs) during ovarian follicular development. We have previously shown that miR-202-5p displays a stage-dependent expression pattern in GCs from goose follicles of different sizes, suggesting that this miRNA could be involved in the regulation of the functions of goose GCs; therefore, in this study, the effects of miR-202-5p on lipid metabolism and steroidogenesis in goose hierarchical follicular GCs (hGCs), as well as its mechanisms of action, were evaluated. Oil Red O staining and analyses of intracellular cholesterol and triglyceride contents showed that the overexpression of miR-202-5p significantly inhibited lipid deposition in hGCs; additionally, miR-202-5p significantly inhibited progesterone secretion in hGCs. A bioinformatics analysis and luciferase reporter assay indicated that Acyl-CoA synthetase long-chain family member 3 (), which activates long-chain fatty acids for the synthesis of cellular lipids, is a potential target of miR-202-5p. ACSL3 silencing inhibited lipid deposition and estrogen secretion in hGCs. These data suggest that miR-202-5p exerts inhibitory effects on lipid deposition and steroidogenesis in goose hGCs by targeting the gene.

摘要

微小RNA（miRNAs）在卵巢卵泡发育过程中对颗粒细胞（GCs）的类固醇生成至关重要。我们之前已经表明，miR-202-5p在不同大小鹅卵泡的颗粒细胞中呈现出阶段依赖性表达模式，这表明该微小RNA可能参与了鹅颗粒细胞功能的调节；因此，在本研究中，评估了miR-202-5p对鹅分级卵泡颗粒细胞（hGCs）脂质代谢和类固醇生成的影响及其作用机制。油红O染色以及细胞内胆固醇和甘油三酯含量分析表明，miR-202-5p的过表达显著抑制了hGCs中的脂质沉积；此外，miR-202-5p显著抑制了hGCs中的孕酮分泌。生物信息学分析和荧光素酶报告基因检测表明，酰基辅酶A合成酶长链家族成员3（ACSL3），其激活长链脂肪酸用于细胞脂质合成，是miR-202-5p的一个潜在靶点。ACSL3基因沉默抑制了hGCs中的脂质沉积和雌激素分泌。这些数据表明，miR-202-5p通过靶向ACSL3基因对鹅hGCs中的脂质沉积和类固醇生成发挥抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/7d1a57dfa6cc/animals-13-00325-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/adf223902940/animals-13-00325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/843f8488d419/animals-13-00325-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/97f59f988d12/animals-13-00325-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/67eb8a99f38e/animals-13-00325-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/bfa2b1e55479/animals-13-00325-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/7d1a57dfa6cc/animals-13-00325-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/adf223902940/animals-13-00325-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/843f8488d419/animals-13-00325-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/97f59f988d12/animals-13-00325-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/67eb8a99f38e/animals-13-00325-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/bfa2b1e55479/animals-13-00325-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9c2/9913746/7d1a57dfa6cc/animals-13-00325-g006.jpg

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