Nicovich Philip R, Walsh James, Böcking Till, Gaus Katharina
ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales, Sydney, New South Wales, Australia.
EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
PLoS One. 2017 Mar 16;12(3):e0173879. doi: 10.1371/journal.pone.0173879. eCollection 2017.
Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the power requirements and combining multiple units into a single fiber launch expands their capability across the required spectral range. We present the NicoLase, an open-source diode laser combiner, fiber launch, and software sequence controller for fluorescence microscopy and super-resolution microscopy applications. Two configurations are described, giving four or six output wavelengths and one or two single-mode fiber outputs, with all CAD files, machinist drawings, and controller source code openly available.
现代荧光显微镜需要在很宽波长范围内具备高功率且由软件控制的照明源。二极管激光器满足功率要求，将多个单元组合到单个光纤发射器中可扩展其在所需光谱范围内的能力。我们展示了NicoLase，这是一种用于荧光显微镜和超分辨率显微镜应用的开源二极管激光组合器、光纤发射器及软件序列控制器。文中描述了两种配置，可提供四个或六个输出波长以及一个或两个单模光纤输出，所有CAD文件、机械图纸和控制器源代码均公开可用。
