Barberis-Maino L, Berger-Bächi B, Weber H, Beck W D, Kayser F H
Institute of Medical Microbiology, University of Zürich, Switzerland.
Gene. 1987;59(1):107-13. doi: 10.1016/0378-1119(87)90271-x.
We present the nucleotide sequence of IS431, a new staphylococcal insertion sequence-like element flanking the mercury-resistance determinant of pI524 and associated with the methicillin-resistance determinant. IS431 left is 800 bp long and has a perfect terminal inverted repeat (IR) of 22 bp; IS431 right is 786 bp long and has a terminal IR homologous to the IR of IS431 left except that the terminal 8 bp are absent. Both IRs share a 10-bp homology with the IR of IS26 from Proteus vulgaris. No directly repeated sequences were detected immediately adjacent to the IRs. An open reading frame (ORF) of 675 bp spans most of the IS431 sequence. Its deduced amino acid (aa) sequence shows 40% homology to the 234-aa-long putative transposase coded by ORFI of IS26.
我们展示了IS431的核苷酸序列，它是一种新的葡萄球菌插入序列样元件，位于pI524的汞抗性决定簇侧翼，并与甲氧西林抗性决定簇相关。IS431左侧长800 bp，有一个22 bp的完美末端反向重复序列（IR）；IS431右侧长786 bp，有一个与IS431左侧的IR同源的末端IR，但末端8 bp缺失。两个IR与普通变形杆菌的IS26的IR有10 bp的同源性。在IR紧邻处未检测到直接重复序列。一个675 bp的开放阅读框（ORF）跨越了大部分IS431序列。其推导的氨基酸（aa）序列与IS26的ORFI编码的234个氨基酸长的推定转座酶显示出40%的同源性。
