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新型原核移动遗传元件IS26的核苷酸序列

Nucleotide sequence of IS26, a new prokaryotic mobile genetic element.

作者信息

Mollet B, Iida S, Shepherd J, Arber W

出版信息

Nucleic Acids Res. 1983 Sep 24;11(18):6319-30. doi: 10.1093/nar/11.18.6319.

Abstract

The DNA sequence of a new IS element, the IS26, is 820 bp long and carries 14 bp perfect terminal inverted repeats. Upon integration, IS26 generates an 8 bp duplication of its target sequence. A large open reading frame within IS26 could code for a protein of 234 amino acids. On its reverse strand, IS26 also carries one large open reading frame, 591 bp long, which contains no stop codon within IS26.

摘要

一种新的插入序列IS26的DNA序列长度为820碱基对,带有14个碱基对的完美末端反向重复序列。整合时,IS26会使其靶序列产生8个碱基对的重复。IS26内的一个大的开放阅读框可编码一个含234个氨基酸的蛋白质。在其反向链上,IS26还带有一个长591碱基对的大开放阅读框,在IS26内没有终止密码子。

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