Chongqing Key Laboratory of Ultrasound Molecular Imaging & State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing and the Ministry of Science and Technology, Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, PR China.
State Key Laboratory of High Performance Ceramic and Superfine Microstructures, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, PR China.
Biomaterials. 2017 Jun;128:84-93. doi: 10.1016/j.biomaterials.2017.03.004. Epub 2017 Mar 8.
The development of efficient strategies for the magnetic hyperthermia ablation of tumors remains challenging. To overcome the significant safety limitations, we developed a thermally contractible, injectable and biodegradable material for the minimally invasive and highly efficient magnetic hyperthermia ablation of tumors. This material was composed of hydroxypropyl methyl cellulose (HPMC), polyvinyl alcohol (PVA) and FeO. The thermal contractibility of HPMC/FeO was designed to avoid damaging the surrounding normal tissue upon heating, which was confirmed by visual inspection, ultrasound imaging and computed tomography (CT). The efficient injectability of HPMC/FeO was proven using a very small needle. The biosafety of HPMC/FeO was evaluated by MTT and biochemical assays as well as flow cytometry (FCM). All the aforementioned data demonstrated the safety of HPMC/FeO. The results of in vitro and ex vivo experiments showed that the temperature and necrotic volume of excised bovine liver were positively correlated with the HPMC/FeO weight, iron content and heating duration. The in vivo experimental results showed that the tumors could be completely ablated using 0.06 ml of HPMC/60%FeO after 180 s of induction heating. We believe that this novel, safe and biodegradable material will promote the rapid bench-to-bed translation of magnetic hyperthermia technology, and it is also expected to bring a new concept for the biomaterial research field.
高效的肿瘤磁热疗消融策略的发展仍然具有挑战性。为了克服重大的安全限制，我们开发了一种可热收缩、可注射和可生物降解的材料，用于肿瘤的微创和高效磁热疗消融。这种材料由羟丙基甲基纤维素（HPMC）、聚乙烯醇（PVA）和 FeO 组成。HPMC/FeO 的热收缩性旨在避免加热时对周围正常组织造成损害，这一点通过肉眼观察、超声成像和计算机断层扫描（CT）得到了证实。通过使用非常小的针头，证明了 HPMC/FeO 的高效可注射性。通过 MTT 和生化分析以及流式细胞术（FCM）评估了 HPMC/FeO 的生物安全性。所有上述数据都证明了 HPMC/FeO 的安全性。体外和体内实验的结果表明，牛肝切除后的温度和坏死体积与 HPMC/FeO 的重量、铁含量和加热时间呈正相关。体内实验结果表明，在 180 秒的诱导加热后，使用 0.06ml 的 HPMC/60%FeO 可以完全消融肿瘤。我们相信，这种新型、安全且可生物降解的材料将促进磁热疗技术的快速从实验室到临床的转化，也有望为生物材料研究领域带来新的概念。
