Stuchlíková Raisová Lucie, Podlipná Radka, Szotáková Barbora, Syslová Eliška, Skálová Lenka
Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University in Prague, Heyrovského 1203, 500 05 Hradec Králové, Czech Republic.
Laboratory of Plant Biotechnology, Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, 165 02 Praha 6 - Lysolaje, Czech Republic.
Ecotoxicol Environ Saf. 2017 Jul;141:37-42. doi: 10.1016/j.ecoenv.2017.03.014. Epub 2017 Apr 10.
Albendazole (ABZ) is a benzimidazole anthelmintic widely used especially in veterinary medicine. Along with other drugs, anthelmintics have become one of a new class of micro-pollutants that disturb the environment but the information about their fate in plants remains limited. The present study was designed to test the uptake and biotransformation of ABZ in the ribwort plantain (Plantago lancelota), a common meadow plant, which can come into contact with this anthelmintic through the excrements of treated animals in pastures. Two model systems were used and compared: cell suspensions and whole plant regenerants. In addition, time-dependent changes in occurrence of ABZ and its metabolites in roots, basal parts of the leaves and tops of the leaves were followed up. Ultrahigh-performance liquid chromatography coupled with high mass accuracy tandem mass spectrometry (UHPLC-MS/MS) led to the identification of 18 metabolites of ABZ formed in the ribwort. In both model systems, the same types of ABZ biotransformation reactions were found, but the spectrum and abundance of the ABZ metabolites detected in cell suspensions and regenerants differed significantly. Cell suspensions seem to be suitable only for qualitative estimations of drug biotransformation reactions while regenerants were shown to represent an adequate model for the qualitative as well as quantitative evaluation of drug uptake and metabolism in plants.
阿苯达唑（ABZ）是一种苯并咪唑类驱虫药，广泛应用于兽医医学领域。与其他药物一样，驱虫药已成为一类新型微污染物，对环境造成干扰，但关于它们在植物中的归宿信息仍然有限。本研究旨在测试阿苯达唑在长叶车前（Plantago lancelota）中的吸收和生物转化情况，长叶车前是一种常见的草地植物，可通过牧场中接受治疗动物的粪便接触到这种驱虫药。使用并比较了两个模型系统：细胞悬浮液和整株再生植株。此外，还跟踪了阿苯达唑及其代谢产物在根、叶基部和叶尖中的随时间变化情况。超高效液相色谱与高精度串联质谱联用（UHPLC-MS/MS）技术鉴定出了长叶车前中形成的18种阿苯达唑代谢产物。在两个模型系统中，均发现了相同类型的阿苯达唑生物转化反应，但在细胞悬浮液和再生植株中检测到的阿苯达唑代谢产物的种类和丰度存在显著差异。细胞悬浮液似乎仅适用于药物生物转化反应的定性评估，而再生植株被证明是用于植物中药物吸收和代谢定性及定量评估的合适模型。
