Nakano T, Hanasaki K, Arita H
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka.
J Biochem. 1987 Oct;102(4):677-80. doi: 10.1093/oxfordjournals.jbchem.a122104.
The rapid Ca2+ increase shown by quin2-loaded platelets was found to be an artifact, probably due to light scattering elicited by collagen. Further findings as to fura2-loaded platelets offered additional support, demonstrating that the initial activation of phospholipase A2 (PLA2) does not require cytoplasmic Ca2+ mobilization. A possible role of the Na+/H+ antiporter as a trigger for collagen-induced activation of PLA2 in rat platelets was presented for the first time.
结果发现，喹啉2负载的血小板所显示的快速Ca2+增加是一种假象，可能是由胶原蛋白引发的光散射所致。关于fura2负载血小板的进一步研究结果提供了更多支持，表明磷脂酶A2（PLA2）的初始激活并不需要细胞质Ca2+动员。首次提出了Na+/H+反向转运体作为大鼠血小板中胶原蛋白诱导的PLA2激活触发因素的可能作用。
