Sakuta Yu, Tsunoda Kin-Ichi, Sato Kiichi
School of Science and Technology, Gunma University.
Anal Sci. 2017;33(3):391-394. doi: 10.2116/analsci.33.391.
Here, we developed a multichannel dialysis microchip having three sets of dialysis systems, which consisted of three independent circulation channels with a common micropump. Each dialysis system was composed of a pneumatic micropump (heart), dialysis unit (renal corpuscle), and cell culture chamber (drug target) as well as circulation and dialysate channels to mimic the circulation system. Small molecules were successfully separated in parallel from macromolecules at the dialysis components. Anticancer tests using this microchip showed results that considered both serum protein-binding nature and drug activity. In the anticancer bioassay, the multichannel chip showed similar reproducible and reliable results as those of the single-channel system but with higher throughput.
在此，我们开发了一种具有三套透析系统的多通道透析微芯片，该透析系统由带有一个共用微型泵的三个独立循环通道组成。每个透析系统由一个气动微型泵（心脏）、透析单元（肾小体）、细胞培养室（药物靶点）以及用于模拟循环系统的循环和透析液通道组成。在透析组件处，小分子成功地与大分子并行分离。使用该微芯片进行的抗癌测试显示的结果兼顾了血清蛋白结合特性和药物活性。在抗癌生物测定中，多通道芯片显示出与单通道系统相似的可重复且可靠的结果，但通量更高。
