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一种用于体外肠道组织研究的间期微流控培养系统。

An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue.

作者信息

Baydoun Martha, Treizeibré Anthony, Follet Jérôme, Vanneste Sadia Benamrouz, Creusy Colette, Dercourt Lucie, Delaire Baptiste, Mouray Anthony, Viscogliosi Eric, Certad Gabriela, Senez Vincent

机构信息

Univ. Lille, CNRS, ISEN-YNCREA, UMR 8520-IEMN, F-59000 Lille, France.

ISA-YNCREA Hauts de France, F-59000 Lille, France.

出版信息

Micromachines (Basel). 2020 Jan 30;11(2):150. doi: 10.3390/mi11020150.

DOI:10.3390/mi11020150
PMID:32019215
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7074597/
Abstract

Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.

摘要

体外组织块培养模型为研究组织生长、更新和疾病背后的复杂机制提供了独特的特性。一个主要缺点是其存活时间短，这取决于活检来源和制备方案。我们描述了一种用于培养全层小鼠结肠组织块的相间微流控培养系统，该系统可使组织的形态结构保持长达192小时。该系统由位于多孔膜顶部的中央孔组成，多孔膜由微通道结构支撑。微流控灌注允许冲洗浆膜侧，同时防止绒毛浸没。八天后，高达33%的样本未显示组织学异常。氧气和葡萄糖传输的数值模拟提供了改进微型设备功能的技术解决方案。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/39c5b79b1315/micromachines-11-00150-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/6bf4348c356c/micromachines-11-00150-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/1b8921bab5e1/micromachines-11-00150-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/f7fce90e2668/micromachines-11-00150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/bc9fd8cc0190/micromachines-11-00150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/118ea2450359/micromachines-11-00150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/01ffe3b9ac93/micromachines-11-00150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/d6e4649e58ae/micromachines-11-00150-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/39c5b79b1315/micromachines-11-00150-g006a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/6bf4348c356c/micromachines-11-00150-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/1b8921bab5e1/micromachines-11-00150-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/f7fce90e2668/micromachines-11-00150-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/bc9fd8cc0190/micromachines-11-00150-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/118ea2450359/micromachines-11-00150-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/01ffe3b9ac93/micromachines-11-00150-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/d6e4649e58ae/micromachines-11-00150-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d5eb/7074597/39c5b79b1315/micromachines-11-00150-g006a.jpg

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