Sharma Madhu, Chadha Pooja
Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Environ Sci Pollut Res Int. 2017 Apr;24(12):11331-11339. doi: 10.1007/s11356-017-8759-1. Epub 2017 Mar 16.
The present study investigated the intertissue differences in genotoxicity induction in the fish, Channa punctatus, in response to 4-nonylphenol. The lethal concentration of 50 (LC) was estimated using a semi-static system. Fish were subjected to three sublethal concentrations of 4-nonylphenol (NP) for 24, 48, 72, and 96 h. Liver, gill, and kidney tissues were analyzed, and the genotoxicity was estimated using the micronucleus test and the comet assay. The frequency of micronucleated cells (MNCs), binucleated cells (BNCs), aberrant cells, and tail moment (TM) increased significantly. The time for maximum induction of genotoxicity for all the parameters considered in the micronucleus assay was 72 h of exposure, whereas the TM was highest at 24 h of exposure followed by a decline. The gill and liver showed higher genotoxicity than kidney tissue demonstrating organ-specific susceptibilities to NP in fish C. punctatus.
本研究调查了斑鳢（Channa punctatus）在接触4-壬基酚后组织间遗传毒性诱导的差异。使用半静态系统估计半数致死浓度（LC50）。将鱼暴露于三种亚致死浓度的4-壬基酚（NP）中24、48、72和96小时。对肝脏、鳃和肾脏组织进行分析，并使用微核试验和彗星试验估计遗传毒性。微核细胞（MNCs）、双核细胞（BNCs）、异常细胞的频率和尾矩（TM）显著增加。微核试验中所有参数的遗传毒性最大诱导时间为暴露72小时，而TM在暴露24小时时最高，随后下降。鳃和肝脏显示出比肾脏组织更高的遗传毒性，表明斑鳢对NP具有器官特异性易感性。
