Mahowald A P, Tiefert M
Department of Biology, Marquette University, Milwaukee, Wisconsin.
Wilhelm Roux Arch Entwickl Mech Org. 1970 Mar;165(1):8-25. doi: 10.1007/BF00576994.
H-uridine was injected into the abdomen ofD. melanogaster andD. immigrans and after 10, 30, 60 and 120 min of incorporation, the ovaries were prepared for autoradiography. The oocyte nucleus was found to synthesize RNA during a short period of vitellogenesis (stage 10A). Ultrastructural studies of the oocyte nucleus were made at the stage active in RNA synthesis and many electron-dense structures were found to appear at this time. Since none of these structures resembled nucleoli in fine structure, it is suggested that the RNA synthesized is non-ribosomal. Other ultrastructural modifications of the oooyte nucleus are presented and discussed.
将H-尿苷注入黑腹果蝇和拟果蝇的腹部，在掺入10、30、60和120分钟后，制备卵巢用于放射自显影。发现卵母细胞核在卵黄发生的短时期（10A期）合成RNA。在RNA合成活跃的阶段对卵母细胞核进行了超微结构研究，发现此时出现了许多电子致密结构。由于这些结构在精细结构上均与核仁不同，因此表明合成的RNA是非核糖体RNA。还介绍并讨论了卵母细胞核的其他超微结构变化。
