Meng Claudia
Zoologisches Institut (I) der Universität Würzburg, Deutschland.
Heiligenberg-Institut für experimentelle Biologie, Heiligenberg, Baden.
Wilhelm Roux Arch Entwickl Mech Org. 1970 Mar;165(1):35-52. doi: 10.1007/BF00576996.
During the longitudinal growth of the oocytes ofPimpla the voluminous oosome area has considerable amounts of newly synthetized proteins (= 1st stage of oosome formation). Short time incubations result in labelling the oosome area as distinctly as the plasm in the nurse cells, in spite of the considerable distance between nurse cells and oosome area. Thus there is no evidence of trophocytic proteins being transported towards the oosome. Consequently during the 1st stage of oosome formation more proteins are synthetized in the oosome area than in the ooplasm.All over the oocyte, oocytic and extra-oocytic components are adding to the propagation of ooplasm (intussusceptive growth). During the 1st stage of its formation, the oosome could possibly contain an additional system for appositional growth within the posterior part of the oocyte, or/and the proteins synthetized herein could play a part in the synthesis of fats. Further questions in connection with these preliminary hypotheses can be answered by more refined methods only. During the oocytic stages following the degeneration of the nurse cell chamber (= 2nd stage of oosome formation), the ever more ball-shaped oosome accumulates trophocytic RNA, which is present all over the rest of the egg space as well. This stable RNA is meant to serve either for the somatic embryogenesis or the germ line.Further autoradiographic observations of the ovarioles essentially confirm known facts concerning the extra-oocytic supply of the oocyte as stated for meroistic ovarioles. This includes the supply with stable and unstable RNA from the nurse cell chamber and proteins from haemolymph for vitellogenesis as well as nurse cell plasm rich in RNA, available during the degeneration of the nurse cell chamber.
在扁股小蜂卵母细胞的纵向生长过程中，体积庞大的卵球体区域有大量新合成的蛋白质（=卵球体形成的第一阶段）。尽管营养细胞与卵球体区域之间距离较远，但短时间孵育后，卵球体区域的标记与营养细胞中的细胞质一样明显。因此，没有证据表明营养细胞的蛋白质向卵球体运输。所以在卵球体形成的第一阶段，卵球体区域合成的蛋白质比卵质中更多。在整个卵母细胞中，卵母细胞和卵母细胞外的成分都在促进卵质的增殖（内填性生长）。在其形成的第一阶段，卵球体可能在卵母细胞后部包含一个额外的附加性生长系统，或者/并且在此合成的蛋白质可能在脂肪合成中起作用。只有通过更精细的方法才能回答与这些初步假设相关的进一步问题。在营养细胞腔退化后的卵母细胞阶段（=卵球体形成的第二阶段），越来越呈球形的卵球体积累营养细胞的RNA，这种RNA在卵的其余空间也都存在。这种稳定的RNA要么用于体细胞胚胎发生，要么用于种系。对卵巢管的进一步放射自显影观察基本上证实了关于多滋式卵巢管中所述的卵母细胞外源性供应的已知事实。这包括从营养细胞腔供应稳定和不稳定的RNA，以及为卵黄生成供应来自血淋巴的蛋白质，还有在营养细胞腔退化期间可获得的富含RNA的营养细胞质。
