Žnidarić Danica, Lui Ante
Department of Zoology, Faculty of Sciences and Department of Histology, School of Veterinary Medicine, University of Zagreb, Croatia.
Wilhelm Roux Arch Entwickl Mech Org. 1969 Dec;162(4):374-383. doi: 10.1007/BF00578702.
Asexual, non-budding hydras were treated in the 1∶75000 solution of E 39 solubile (Bayer). They were feeding, growing and budding for six days. The interstitial cells found in the ectoderm at the moment of treatment differentiated into cnidoblasts during that period. The cells that happened to be in the gastroderm at that time, differentiated into interstitial cells which were not able to cross the mesoglea due to the E 39 activity. In the gastroderm a great number of cnids appeared.These observations support the hypothesis that in normal hydra the interstitial cells formed by the dedifferentiation of gland cells pass into the ectoderm. There they are transformed into cnidoblasts which pass into the mesoglea. Prom the gastroderm the cnidoblasts come into the ectoderm of the tentacles through endodermal cells and the coelenteric fluid.
将无性、不形成芽体的水螅置于1∶75000的E 39可溶物(拜耳公司)溶液中处理。它们进食、生长并形成芽体达六天之久。处理时在外胚层发现的间质细胞在此期间分化为刺细胞。当时处于内胚层的细胞则分化为间质细胞,由于E 39的作用,这些间质细胞无法穿过中胶层。在内胚层出现了大量刺丝囊。这些观察结果支持了这样一种假说:在正常水螅中,由腺细胞去分化形成的间质细胞进入外胚层。在那里它们转化为刺细胞,然后进入中胶层。刺细胞从中胶层通过内胚层细胞和肠腔液进入触手的外胚层。