Barber Mary Lee, Mead James F
Department of Biology, California State University, 91324, Northridge, California, USA.
Laboratory of Nuclear Medicine and Radiation Biology, UCLA, 90024, Los Angeles, California, USA.
Wilehm Roux Arch Dev Biol. 1975 Mar;177(1):19-27. doi: 10.1007/BF00848624.
Lipids from surface ghosts, prepared from sea urchin eggs before and after fertilization, differ in the relative proportions of neutral and polar lipids. Percents of triglyceride and phosphatidyl serine or inositol increase while percent phosphatidyl choline decrease after fertilization. Fatty acids from GLC of lipid fractions (triglycerides, free fatty acids, phosphatidyl ethanolamine, phosphatidyl choline, and phosphatidyl serine or inositol) are characteristic for each fraction, and differ quantitatively in ghosts before and after fertilization. Fatty acids of unfertilized ghost lipids are in general longer chain and more unsaturated than those of the fertilized. Possible effects of these lipid changes on surface characteristics are discussed.
受精前后从海胆卵制备的表面空壳中的脂质，其中性脂质和极性脂质的相对比例有所不同。受精后，甘油三酯以及磷脂酰丝氨酸或肌醇的百分比增加，而磷脂酰胆碱的百分比降低。脂质组分（甘油三酯、游离脂肪酸、磷脂酰乙醇胺、磷脂酰胆碱以及磷脂酰丝氨酸或肌醇）的气相色谱法分析得到的脂肪酸对每个组分而言都具有特征性，并且在受精前后的空壳中存在数量差异。未受精空壳脂质的脂肪酸一般比受精后的脂肪酸链更长且不饱和程度更高。文中讨论了这些脂质变化对表面特性可能产生的影响。
