French Vernon
Developmental Biology Group, University of Sussex, Brighton, Sussex, UK.
Division of Developmental Biology, National Institute for Medical Research, The Ridgeway Mill Hill, NW7 1AA, London.
Wilehm Roux Arch Dev Biol. 1976 Mar;179(1):57-76. doi: 10.1007/BF00857640.
The interactions occuring between graft and host leg epidermis at a congruent junction (non-rotated, homopleural combination of components cut perpendicular to the proximal-distal axis) were studied at the tibia level in the cockroach,Blattella germanica. Grafts were made between dark (Bl) and light (br) cuticle colour mutants. 1) Precise boundaries could not usually be drawn between Bl and br tissue over areas of bare cuticle but spines, hairs and claws could be identified, providing a good indication of the graft or host origin of regenerated structures. 2) When the graft tarsus remained intact, segmented structures were not regenerated from the junction. Host distal tibia and graft proximal tibia interacted to produce a reversed orientation intercalary regenerate, usually composed mainly of host cells which had become more proximal than their level of origin. 3) When the graft tarsus was amputated (or broken off), nearly 50% of congruent junctions regenerated segmented distal structures, which were classified as "autonomous" or "lateral". Amputation of the graft tarsus acted, not through removal of any inhibition, but by hindering healing of the junction because of the apolysis of graft tibial epidermis. 4) Distal structures regenerated autonomously by host and graft components of the junction were either complete or partial (fused at a common level in the tarsus). 5) Lateral regenerates were of joint origin and usually distally incomplete. They were stable and, when amputated, were regenerated to approximately the same level, in the presence or absence of the graft tarsus. 6) It is concluded that autonomous regeneration occurred from junctions which had totally failed to heal, and that lateral regeneration occurred from an unhealed sector of a junction. Laterals were therefore regenerated from a bilaterally symmetrical, partial circumference. They are compared to other incomplete regenerates found in analogous situations. The relationship between transverse organization and distal incompleteness is obscure. 7) Segmented structures are thus regenerated only in situations where host and graft do not heal and interact (at least initially) over all or part of the circumference of the junction: interaction results in the formation of an unsegmented intercalary regenerate comprising the levels normally lying between host and graft on the proximal-distal axis.
在德国小蠊的胫骨水平，研究了移植物与宿主腿部表皮在全等连接处（非旋转，垂直于近端 - 远端轴切割的同胸膜成分组合）之间发生的相互作用。移植物取自深色（Bl）和浅色（br）角质层颜色突变体之间。1）在裸露角质层区域，通常无法在Bl和br组织之间划定精确边界，但可以识别刺、毛和爪，这为再生结构的移植物或宿主来源提供了很好的指示。2）当移植物跗节保持完整时，连接处不会再生出分段结构。宿主远端胫骨和移植物近端胫骨相互作用产生反向定向的间插再生体，通常主要由比其起源水平更靠近近端的宿主细胞组成。3）当移植物跗节被截肢（或折断）时，近50%的全等连接处再生出分段的远端结构，这些结构被分类为“自主的”或“侧向的”。移植物跗节的截肢作用不是通过消除任何抑制，而是由于移植物胫骨表皮的脱溶作用阻碍了连接处的愈合。4）由连接处的宿主和移植物成分自主再生的远端结构要么是完整的，要么是部分的（在跗节的共同水平融合）。5）侧向再生体起源于关节，通常在远端不完整。它们是稳定的，并且在截肢时，无论移植物跗节是否存在，都会再生到大致相同的水平。6）得出的结论是，自主再生发生在完全未能愈合的连接处，而侧向再生发生在连接处未愈合的部分区域。因此，侧向再生体从双侧对称的部分圆周再生而来。将它们与在类似情况下发现的其他不完全再生体进行了比较。横向组织与远端不完全性之间的关系尚不清楚。7）因此，分段结构仅在宿主和移植物不愈合且在连接处的全部或部分圆周上（至少最初）相互作用的情况下再生：相互作用导致形成一个不分段的间插再生体，其包含近端 - 远端轴上通常位于宿主和移植物之间的水平。
