Tucker Gail Susan, Wyttenbach Charles R
Department of Physiology and Cell Biology, University of Kansas, 66044, Lawrence, Kansas, U.S.A.
Marine Biological Laboratory, 02543, Woods Hole, Massachusetts, U.S.A.
Wilehm Roux Arch Dev Biol. 1975 Sep;177(3):163-181. doi: 10.1007/BF00848078.
A comparison of two genetic stocks ofCampanularia flexuosa revealed consistent differences with respect to (1) the number of eggs present in the maturefemale reproductive body (gonangium), (2) the level on each upright stem at which the first gonangium forms, and (3) their preference for the formation of reproductive versusvegetative (side-branch) structures.Alterations in food intake established the optimum feeding level for maximal production of structurally normal gonangia and also showed differences between stocks in the effects of reduced food intake on gonangial versus vegetative growth.A staging series for gonangiogenesis was developed from observations of filling of the gonangium with primordial egg cells (PEC), gonangial elongation, funnel maturation and oogenesis.When upright segments bearing immature gonangia are isolated from the stolon the gonangia frequently resorb, transform into vegetative outgrowths, or form structurally abnormal gonangia bearing fewer germ cells than normal. These responses, and the observed accumulation of PEC at sites of presumptive gonangial outgrowth, support a stolonic origin hypothesis for PEC and implicate PEC and oocytes in the initiation, maintenance and maturation of structurally normal gonangia. In addition, continued differentiation of the gonangial structure appears to be necessary for oocyte maturation.Possible mechanisms for somatic and gametic cell recruitment, and the theoretical importance of cellular proliferation, aging, cell-shape changes, cell movement and cellular interactions in normal gonangial differentiation are discussed.
（1）成熟雌生殖体（生殖窠）中存在的卵的数量；（2）第一个生殖窠在每个直立茎上形成的水平；（3）它们对形成生殖结构与营养（侧枝）结构的偏好。食物摄入量的改变确定了产生结构正常生殖窠的最大产量的最佳摄食水平，并且还显示了株系之间在食物摄入量减少对生殖窠生长与营养生长的影响方面的差异。根据对生殖窠中原始卵细胞（PEC）的填充、生殖窠伸长、漏斗成熟和卵子发生的观察，建立了生殖窠发生的分期系列。当从匍匐茎分离出带有未成熟生殖窠的直立节段时，生殖窠经常吸收、转化为营养生长物，或形成结构异常且生殖细胞比正常情况少的生殖窠。这些反应，以及在假定的生殖窠生长部位观察到的PEC积累，支持了PEC的匍匐茎起源假说，并暗示PEC和卵母细胞在结构正常的生殖窠的起始、维持和成熟中起作用。此外，生殖窠结构的持续分化似乎是卵母细胞成熟所必需的。讨论了体细胞和配子细胞募集的可能机制，以及细胞增殖、衰老、细胞形状变化、细胞运动和细胞相互作用在正常生殖窠分化中的理论重要性。
