Géraudie Jacqueline
Laboratoire d'Anatomie Comparée, Université Paris VII, France.
Wilhelm Roux Arch Entwickl Mech Org. 1974 Sep;175(3):221-241. doi: 10.1007/BF00582093.
In a study with the light microscope, we investigated the tissular localization of glycogen, R.N.A., and 4 phosphohydrolases in the pelvic fin bud of two species of Trout,Salmo fario andSalmo gairdneri, Pisces, Teleostei. In both, the epiderm bud, including the "pseudo-apical cap", is devoid of glycogen and the amount of R.N.A. histochemically demonstrable is not greater than in epidermal regions outside the bud. InSalmo gairdneri but not inSalmo fario, we observed four phosphatases, only in the inner layer of the epiderm of the fin bud: alkaline phosphomonoesterases, naphthol AS-BI acid phosphatase, nonspecific ATPase and AMPase. The "initial mesenchyme", from the somatopleura's proliferation, contains a great quantity of R.N.A. In both species we found there the four enzymes. The "secondary mesenchyme", obtained from the dispersion of four ventral somitic processes, has a metabolism that is similar inSalmo fario andSalmo gairdneri; alkaline phosphomonoesterase is found only inSalmo gairdneri.
在一项光学显微镜研究中，我们调查了两种鳟鱼（鲑科鱼类，硬骨鱼纲）——法罗鲑（Salmo fario）和吉氏鲑（Salmo gairdneri）——臀鳍芽中糖原、RNA和4种磷酸水解酶的组织定位。在这两种鱼中，表皮芽，包括“假顶端帽”，都没有糖原，并且通过组织化学方法可检测到的RNA量并不比芽外的表皮区域多。在吉氏鲑中，而不是在法罗鲑中，我们仅在鳍芽表皮的内层观察到四种磷酸酶：碱性磷酸单酯酶、萘酚AS-BI酸性磷酸酶、非特异性ATP酶和AMP酶。由体壁层增殖形成的“原始间充质”含有大量RNA。在这两个物种中我们都发现了这四种酶。从四个腹侧体节突起分散形成的“次级间充质”，在法罗鲑和吉氏鲑中的代谢相似；碱性磷酸单酯酶仅在吉氏鲑中发现。
