保幼激素对家蟋蟀胚胎发育过程中DNA合成的影响。

Effect of juvenile hormone on DNA synthesis during embryogenesis inAcheta domesticus.

Rao K D P, Krishnakumaran A

Department of Biology, Marquette University, 53233, Milwaukee, Wisconsin, USA.

Department of Zoology, Delhi University, Delhi 7, India.

Wilhelm Roux Arch Entwickl Mech Org. 1974 Sep;174(3):276-284. doi: 10.1007/BF00573232.

Abstract

The effect of juvenile hormone on embryonic development in the cricket,Acheta domesticus, was investigated. Application of 1 to 2.5 μg of methyl 12,14-dihomojuvenate (cecropia juvenile hormone) in 1 μl of acetone to 7-day-old cricket embryos inhibited their further growth. In such embryos, differentiation of some of the embryonic organs and tissues such as nerves, muscles and cuticular structures continued. However, embryonic growth was arrested, their morphology was abnormal and they failed to hatch. 2) Lipid extracts from adult maleHyalophora cecropia which possess juvenile hormone activity also showed similar effects on embryonic development. But lipid extracts prepared from allatectomized adult maleH. cecropia lacking juvenile hormone activity, did not inhibit embryonic development. 3) DNA synthesis in the embryonic tissues of the JH-treated and control embryos was investigated using an autoradiographic method of determination of H thymidine incorporation into nuclear DNA. The results showed that DNA synthesis in epidermal and mesenchymal cells of the cricket embryo decreased gradually after application of 1 μg of JH to 7-day-old embryos and ceased within 6 days after application of JH. 4) From these observations it is suggested that JH may inhibit embryonic development by suppression of DNA synthesis and cell divisions.

摘要

研究了保幼激素对家蟋蟀胚胎发育的影响。将1至2.5微克的12,14 - 二高保幼激素甲酯（天蚕保幼激素）溶于1微升丙酮中，施用于7日龄的蟋蟀胚胎，抑制了它们的进一步生长。在这些胚胎中，一些胚胎器官和组织如神经、肌肉和表皮结构的分化仍在继续。然而，胚胎生长停滞，形态异常，无法孵化。2) 具有保幼激素活性的成年雄性天蚕蛾的脂质提取物对胚胎发育也有类似影响。但从切除咽侧体的成年雄性天蚕蛾制备的缺乏保幼激素活性的脂质提取物，并未抑制胚胎发育。3) 采用放射自显影法测定³H胸腺嘧啶核苷掺入核DNA的量，研究了保幼激素处理组和对照组胚胎组织中的DNA合成。结果表明，对7日龄胚胎施用1微克保幼激素后，蟋蟀胚胎表皮和间充质细胞中的DNA合成逐渐减少，并在施用保幼激素后6天内停止。4) 从这些观察结果推测，保幼激素可能通过抑制DNA合成和细胞分裂来抑制胚胎发育。