Naruse Shouya, Ogino Mayuko, Nakagawa Takao, Yasuno Yoko, Jouraku Akiya, Shiotsuki Takahiro, Shinada Tetsuro, Miura Ken, Minakuchi Chieka
Applied Entomology Laboratory, Graduate School of Bio-Agricultural Sciences, Nagoya University, Furocho, Chikusa, Nagoya 464-8601, Japan.
Graduate School of Science, Osaka City University, Osaka 558-8585, Japan.
J Pestic Sci. 2021 Feb 20;46(1):60-67. doi: 10.1584/jpestics.D20-075.
Insect juvenile hormone (JH) mimics (JHMs) are known to have ovicidal effects if applied to adult females or eggs. Here, we examined the effects of exogenous JHMs on embryonic development of the bean bug, . The expression profiles of JH early response genes and JH biosynthetic enzymes indicated that JH titer was low for the first 3 days of the egg stage and increased thereafter. Application of JH III skipped bisepoxide (JHSB) or JHM on Day 0 eggs when JH titer was low caused reduced hatchability, and the embryos mainly arrested in mid- or late embryonic stage. Application of JHMs on Day 5 eggs also resulted in an arrest, but this was less effective compared with Day 0 treatment. Interestingly, ovicidal activity of synthetic JHMs was much lower than that of JHSB. This study will contribute to developing novel insecticides that are selective among insect species.
已知昆虫保幼激素（JH）类似物（JHMs）若施用于成年雌性或卵上会产生杀卵作用。在此，我们研究了外源JHMs对豆蝽胚胎发育的影响。JH早期反应基因和JH生物合成酶的表达谱表明，在卵期的前3天JH滴度较低，此后升高。当JH滴度较低时，在第0天的卵上施用JH III双环氧物（JHSB）或JHMs会导致孵化率降低，胚胎主要停滞在胚胎中期或后期。在第5天的卵上施用JHMs也会导致胚胎停滞，但与第0天的处理相比效果较差。有趣的是，合成JHMs的杀卵活性远低于JHSB。这项研究将有助于开发在昆虫物种中具有选择性的新型杀虫剂。
