Institut de Biologia Evolutiva (CSIC-UPF), Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
Insect Biochem Mol Biol. 2010 Sep;40(9):660-5. doi: 10.1016/j.ibmb.2010.06.006. Epub 2010 Jun 11.
Levels of juvenile hormone III (JH), FGLamide allatostatin peptides (ASTs), ASTs precursor (preproAST) mRNA and methyl farnesoate epoxidase (CYP15A1) mRNA were measured in embryos of the cockroach Blattella germanica. JH starts to rise just after dorsal closure, reaches maximal levels between 60% and 80% of embryogenesis, and decrease subsequently to undetectable levels. ASTs show low levels during the first two thirds of embryogenesis, increase thereafter and maintain high levels until hatching. PreproAST mRNA shows quite high levels during the two days following oviposition, thus behaving as a maternal transcript, the levels then become very low until mid embryogenesis, and increase afterwards, peaking towards the end of embryo development. CYP15A1 transcripts were detected around 25% embryogenesis and the levels tended to increase through embryogenesis, although differences amongst the days studied were not statistically significant. The opposite patterns of JH and AST towards the end of embryo development, along with the detection of AST immunoreactivity in corpora allata from late embryos, suggest that JH decline is caused by the increase of AST. Moreover, the uncorrelated patterns of JH concentration and CYP15A1 mRNA levels suggest that CYP15A1 expression does not modulate JH production.
在德国蟑螂 Blattella germanica 的胚胎中测量了保幼激素 III (JH)、FGL 酰胺神经肽 (ASTs)、AST 前体 (preproAST) mRNA 和甲基法呢酯环氧化物酶 (CYP15A1) mRNA 的水平。JH 在背侧闭合后开始上升，在胚胎发生的 60%到 80%之间达到最高水平，随后下降到无法检测的水平。ASTs 在胚胎发生的前三分之二期间水平较低，此后增加并保持高水平直到孵化。preproAST mRNA 在产卵后两天表现出相当高的水平，因此表现为母源转录本，然后水平非常低，直到胚胎发生中期，然后增加，在胚胎发育结束时达到峰值。CYP15A1 转录本在胚胎发生的 25%左右被检测到，水平随着胚胎发生而趋于增加，尽管研究天数之间的差异没有统计学意义。JH 和 AST 在胚胎发育末期的相反模式，以及晚期胚胎的咽侧体中 AST 免疫反应性的检测，表明 JH 的下降是由 AST 的增加引起的。此外，JH 浓度和 CYP15A1 mRNA 水平的不相关模式表明 CYP15A1 表达不会调节 JH 的产生。
