Cassin C, Capuron A
Laboratoire d'Embryologie Expérimentale, Université des Sciences et Techniques de Lille, B.P. 36, 59650, Villeneuve d'Ascq, France.
Wilehm Roux Arch Dev Biol. 1977 Jun;181(2):107-112. doi: 10.1007/BF00848435.
Transplantations into the blastocoel of gastrulae and cultures in vitro of the stomodeal region ofPleurodeles waltlii Michah embryos have been carried out. These experiments gave the following results:Neural crest cells reach the stomodeal region at the young tail-bud stage (stage 22), and are able to take part in formation of teeth and cartilage, it is possible to dissociate the phenomena of mouth opening, complete mouth formation and complete head formation, differentiation of a digestive tube from the pharyngeal endoderm and the formation of a mouth opening both depend on the presence of mesentoderm and mesectoderm in the explant or in the environment, during the tail-bud stage a symmetry factor plays a part in mouth formation, bone formation likewise coincides with the presence of neural crest cells.
已将细胞移植到有尾目动物米氏肋突螈胚胎原口区域的囊胚腔并进行体外培养。这些实验得出了以下结果:神经嵴细胞在幼体尾芽期(第22阶段)到达原口区域,并且能够参与牙齿和软骨的形成,有可能将开口、完整口部形成和完整头部形成的现象分离,咽内胚层分化出消化管以及开口的形成均取决于外植体或环境中中胚层和中外胚层的存在,在尾芽期,一个对称因素在口部形成中起作用,骨形成同样与神经嵴细胞的存在相一致。