Kauffman Stuart Alan, Ling Elizabeth
Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, 19104, Philadelphia, Pennsylvania, USA.
Wilehm Roux Arch Dev Biol. 1980 Jun;189(2):147-153. doi: 10.1007/BF00848503.
Nasobemia (Ns) is a dominant homeotic mutant ofDrosophila melanogaster which converts parts or all of the antenna to mesothoracic leg.Ns has a temperature sensitive period between 48 and 60 h. The hypothesis thatNs acts during this period and is not required thereafter to maintain the homeotic transformation to leg was tested by removingNs fromNs/+ cells at different stages of development through X-ray induced somatic recombination. The expression of theNs homeotic transformation in recombinant wild type (+/+) cells increased sharply between 48 and 65 h. In clones induced after 65 h the expression of the leg transformation was equal in large and small +/+ clones. We interpret these results as supporting the hypothesis that transient action ofNs between 48 and 65 h switches antennal cells to a clonally stable leg determined state whose maintenance does not require futherNs action.
鼻化症（Ns）是黑腹果蝇的一种显性同源异型突变体，它会使部分或全部触角转变为中胸腿。Ns在48至60小时之间有一个温度敏感期。通过X射线诱导的体细胞重组，在发育的不同阶段从Ns/+细胞中去除Ns，以检验Ns在此期间起作用且此后维持向腿的同源异型转变不需要它这一假说。重组野生型（+/+）细胞中Ns同源异型转变的表达在48至65小时之间急剧增加。在65小时后诱导的克隆中，腿转变的表达在大小不同的+/+克隆中是相同的。我们将这些结果解释为支持以下假说：Ns在48至65小时之间的短暂作用将触角细胞转变为克隆稳定的腿决定状态，其维持不需要Ns的进一步作用。
