Keller R, Adelung D
II. Zoologisches Institut der Freien Universität Berlin, Deutschland.
II. Zoologisches Institut der Justus Liebig-Universität Gießen, Deutschland.
Wilhelm Roux Arch Entwickl Mech Org. 1970 Sep;164(3):209-221. doi: 10.1007/BF00582033.
The structural changes in the integumentary tissues during a molt cycle of the crayfishOrconectes limosus and the stages of the molt cycle are described. The incorporation of uridine-5-H into the nuclei of the epidermis and the tegumental glands during the molt cycle was determined by autoradiography and grain counting. In the epidermal nuclei the incorporation reaches a peak immediately before molt; a first, poorly expressed peak seems to be reached in stageD which is characterized by apolysis and the preparation for secretion of the new epicuticle. In the early premolt stages (D, D) the activity of the tegumental gland nuclei compared to that of the epidermal nuclei is higher than in the later premolt stages. A first peak is reached in stage D, a second one of similar size in stage D. In stage D, during and shortly after the secretion of the new epicuticle, heavily labelled hemolymph cells are found in the integumentary tissue beneath the epidermis and between the epidermal cells.Compared toCarcinus maenas only an approximative estimation of the hormone titer could be achieved. This is due to technical difficulties in connection with the very low hormone content of the crayfish. Measurable amounts could be detected between the premolt stages D and D. In this period a rise of the RNA synthesis was observed. The peak of RNA synthesis follows the peak of the molting hormone with a certain delay. This is in accordance with the hypothesis of hormonal control of RNA synthesis during the molt cycle in arthropods. A comparison of the time course of RNA synthesis in the crayfish with the better known time course of the hormone content inCarcinus maenas exhibits a good accordance.
描述了小龙虾奥氏原螯虾蜕皮周期中体表组织的结构变化以及蜕皮周期的各个阶段。通过放射自显影和颗粒计数确定了蜕皮周期中尿苷 - 5 - H掺入表皮细胞核和体壁腺细胞核的情况。在表皮细胞核中，掺入在蜕皮前立即达到峰值；在以表皮与真皮分离和新表皮分泌准备为特征的D期似乎达到第一个表达较弱的峰值。在蜕皮前期早期（D、D），与表皮细胞核相比，体壁腺细胞核的活性高于蜕皮后期。在D期达到第一个峰值，在D期达到第二个大小相似的峰值。在D期，在新表皮分泌期间及之后不久，在表皮下方和表皮细胞之间的体表组织中发现大量标记的血淋巴细胞。与绿蟹相比，只能对激素滴度进行大致估计。这是由于小龙虾激素含量极低带来的技术困难。在蜕皮前期D和D之间可检测到可测量的量。在此期间观察到RNA合成增加。RNA合成峰值在蜕皮激素峰值之后有一定延迟出现。这与节肢动物蜕皮周期中激素控制RNA合成的假说一致。将小龙虾RNA合成的时间进程与绿蟹中更知名的激素含量时间进程进行比较，结果显示出良好的一致性。
