从小龙虾（Jasus lalandei H. Milne-Edwards）中分离蜕皮激素（20R-羟基蜕皮酮）。
Isolation of crustecdysone (20R-hydroxyecdysone) from a crayfish (Jasus lalandei H. Milne-Edwards).
作者信息
Horn D H, Fabbri S, Hampshire F, Lowe M E
出版信息
Biochem J. 1968 Sep;109(3):399-406. doi: 10.1042/bj1090399.
Abstract
- A small amount (2mg.) of crustecdysone, a moulting hormone of crustaceans, was isolated from 1 ton of crayfish waste. 2. The purification procedure used was developed with the aid of crustacean and insect bioassays. 3. CM-Sephadex was found to be superior to Sephadex and very effective for the chromatographic separation of crustecdysone from other non-ionic compounds. The higher efficiency of CM-Sephadex is attributed to the greater number of carboxyl groups available for hydrogen-bonding. 4. Reversed-phase chromatography, with butan-1-ol-cyclohexane mixtures as the stationary phase and water as the flowing phase, proved superior to countercurrent distribution with these solvents for the fractionation of purified extracts. 5. A second moulting hormone, deoxycrustecdysone, and the red-concentrating hormone were obtained in a partially purified form.
摘要
- 从1吨小龙虾废弃物中分离出少量（2毫克）蜕皮激素，一种甲壳类动物的蜕皮激素。2. 所采用的纯化程序是借助甲壳类动物和昆虫生物测定法开发的。3. 发现CM - 葡聚糖凝胶优于葡聚糖凝胶，对于从其他非离子化合物中色谱分离蜕皮激素非常有效。CM - 葡聚糖凝胶效率更高归因于可用于氢键结合的羧基数量更多。4. 以丁醇 - 环己烷混合物为固定相、水为流动相的反相色谱法，在纯化提取物的分馏方面被证明优于用这些溶剂进行的逆流分配法。5. 第二种蜕皮激素，脱氧蜕皮激素，以及红色浓缩激素以部分纯化的形式获得。
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