Steiner Emil, Koller-Wiesinger Maja, Nöthiger Rolf
Zoologisches Institut der Universität Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland.
Wilehm Roux Arch Dev Biol. 1981 May;190(3):156-160. doi: 10.1007/BF00867802.
The transdetermination capacities of leg discs ofDrosophila melanogaster were examined by mechanically disrupting and kneading whole discs from late third instar larvae and by culturing the resulting tissue mass for 10-14 days in adult female abdomens where the cells continued to divide. The grown implants were then dissected from the abdomens and injected into third instar larvae to undergo metamorphosis.After this treatment, prothoracic leg discs ofDrosophila melanogaster transdetermined with a high frequency (59% of all implants) to wing. Mesothoracic leg discs also transdetermined to wing, but at a very low frequency (4%). Metathoracic leg discs exhibited the same low frequency of transdetermination (4%), but in this case the direction of transdetermination was to haltere (Table 1,D. melanogaster).Very similar results were obtained with leg discs ofDrosophila nigromelanica (Table 1,D. nigromelanica), showing that the peculiar behavior of the three leg discs is not unique forDrosophila melanogaster.The homeotic mutation Polycomb (Pc ) which partially transforms meso- and metathoracic legs into prothoracic legs did not significantly increase the frequencies of transdetermination in these leg dises and had clearly no effect on the direction of transdetermination (Table 1).
通过机械破坏和揉捏三龄幼虫后期的完整果蝇腿盘，并将所得组织团块在成年雌性果蝇腹部培养10 - 14天（细胞在此继续分裂），来检测黑腹果蝇腿盘的转决定能力。然后从腹部解剖出生长后的植入物，并注射到三龄幼虫中使其变态。经过这种处理后，黑腹果蝇的前胸腿盘以高频率（所有植入物的59%）转决定为翅。中胸腿盘也转决定为翅，但频率非常低（4%）。后胸腿盘表现出相同的低转决定频率（4%），但在这种情况下，转决定方向是变为平衡棒（表1，黑腹果蝇）。用黑腹黑果蝇的腿盘也得到了非常相似的结果（表1，黑腹黑果蝇），表明这三种腿盘的特殊行为并非黑腹果蝇所独有。同源异型突变体多梳（Pc）会使中胸和后胸腿部分转变为前胸腿，但在这些腿盘中并没有显著提高转决定频率，并且对转决定方向显然没有影响（表1）。
