Fragoulis Emmanuel G, Traub Peter
Department of Biochemistry, Cell and Molecular Biology and Genetics, University of Athens, Panepistemiopolis Kouponia, 157.01, Athens, Greece.
Max Planck Institute for Cell Biology, Rosenhoff, D-6802, Ladenburg, Germany.
Wilehm Roux Arch Dev Biol. 1984 Jan;194(1):25-31. doi: 10.1007/BF00848950.
Crude, cell-free protein-synthesizing systems were prepared from follicles of two different stages of development in the ovariole of the silkmothAntherea pernyi. The efficiency of the translation of natural and synthetic mRNAs in these systems was compared with that in a cell-free wheat germ system. A postmitochondrial extract (S-30) from the follicles almost completely inhibited protein synthesis in a polyribosome-dependent, cell-free systems. A specific ribonuclease, obtained from the post mitochondrial extract by ammonium sulphate precipitation, heat denaturation and DEAE-cellulose chromatography, inhibited polyribosome-dependent protein synthesis. The effect of this specific ribonuclease on the structural integrity of radioactive RNAs and ribosomal subunits, which were isolated from Ehrlich ascites tumor cells, was also studied.
从柞蚕卵巢管中两个不同发育阶段的卵泡制备了粗制的无细胞蛋白质合成系统。将这些系统中天然和合成mRNA的翻译效率与无细胞小麦胚芽系统中的翻译效率进行了比较。卵泡的线粒体后提取物(S-30)几乎完全抑制了多核糖体依赖性无细胞系统中的蛋白质合成。通过硫酸铵沉淀、热变性和DEAE-纤维素色谱法从线粒体后提取物中获得的一种特异性核糖核酸酶抑制了多核糖体依赖性蛋白质合成。还研究了这种特异性核糖核酸酶对从艾氏腹水瘤细胞中分离出的放射性RNA和核糖体亚基结构完整性的影响。