嘌呤霉素使哺乳动物多核糖体解离为亚基。
Dissociation of mammalian polyribosomes into subunits by puromycin.
作者信息
Blobel G, Sabatini D
出版信息
Proc Natl Acad Sci U S A. 1971 Feb;68(2):390-4. doi: 10.1073/pnas.68.2.390.
Abstract
Hepatic ribosomes have been dissociated into biologically active subunits as follows. Polysomes were treated at 0 degrees C with puromycin at high ionic strength. This released most of the nascent polypeptide chains without dissociating the polysomes, which retained the mRNA and the tRNA moiety of peptidyl tRNA, but were unable to continue the translation of mRNA. The polysomes were then heated to 37 degrees C, when they dissociated completely into subunits. Similar treatment without puromycin resulted in only partial dissociation.
摘要
肝核糖体已按如下方式解离成具有生物活性的亚基。多核糖体在0℃下于高离子强度条件下用嘌呤霉素处理。这释放了大部分新生多肽链，而多核糖体并未解离，其保留了mRNA和肽基tRNA的tRNA部分，但无法继续翻译mRNA。然后将多核糖体加热至37℃，此时它们完全解离成亚基。在没有嘌呤霉素的情况下进行类似处理仅导致部分解离。
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本文引用的文献
1
ULTRACENTRIFUGAL STUDIES ON THE DISSOCIATION OF HEPATIC RIBOSOMES.肝脏核糖体解离的超速离心研究
J Mol Biol. 1965 Feb;11:149-65. doi: 10.1016/s0022-2836(65)80047-x.
2
ROLE OF THE GENETIC MESSAGE IN POLYRIBOSOME FUNCTION.遗传信息在多核糖体功能中的作用。
J Mol Biol. 1965 Feb;11:358-72. doi: 10.1016/s0022-2836(65)80063-8.
3
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
4
Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.大鼠肝脏中游离核糖体与膜结合核糖体的研究。II. 核糖体与膜的相互作用。
J Mol Biol. 1967 Jun 14;26(2):293-301. doi: 10.1016/0022-2836(67)90298-7.
5
Factors influencing the puromycin-induced release of protein from liver ribosomes.影响嘌呤霉素诱导肝脏核糖体释放蛋白质的因素。
Biochim Biophys Acta. 1966 Sep;123(3):561-73. doi: 10.1016/0005-2787(66)90223-1.
6
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J Mol Biol. 1969 Jul 14;43(1):151-61. doi: 10.1016/0022-2836(69)90085-0.
7
Purification and properties of dog pancreas ribosomes and subunits.犬胰腺核糖体及其亚基的纯化与性质
Biochemistry. 1969 Aug;8(8):3295-303. doi: 10.1021/bi00836a025.
8
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Biochim Biophys Acta. 1968 Aug 23;166(1):48-57. doi: 10.1016/0005-2787(68)90489-9.
9
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J Mol Biol. 1970 Oct 14;53(1):1-19. doi: 10.1016/0022-2836(70)90042-2.
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J Cell Biol. 1970 Apr;45(1):130-45. doi: 10.1083/jcb.45.1.130.
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