Zagris Nikolas, Matthopoulos Demetrios
Division of Genetics and Cell and Developmental Biology, Department of Biology, University of Patras, Patras, Greece.
Laboratory of General Biology, Medical School, University of Ioannina, Ioannina, Greece.
Rouxs Arch Dev Biol. 1986 Jan;195(1):80-83. doi: 10.1007/BF00444044.
Induction of the primitive streak is correlated with specific qualitative and quantitative changes in protein synthesis in the component areas of chick blastoderm. Blastoderm embryos at the initial to intermediate primitive streak stage were labeled with L-[S] methionine. Radioactively labeled proteins separated by two-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis revealed differences in the number and density of spots among the component areas of the epiblast and hypoblast. Protein patterns of the area opaca, marginal zone and central area of the epiblast are very similar qualitatively but show distinct quantitative differences. A comparison between any of the component areas of the epiblast and the hypoblast in chick blastoderm embryos, however, reveals both qualitative and quantitative differences. A protein with a molecular weight of 30,000 unique to the component areas of the epiblast, and proteins with a molecular weight of 22,000 and 37,000 unique to the hypoblast are prominent and seem to be related to the initial appearance of the primitive streak.
原条的诱导与鸡胚盘各组成区域蛋白质合成的特定定性和定量变化相关。在原条初始至中间阶段的胚盘胚胎用L-[S]甲硫氨酸进行标记。通过二维十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳分离的放射性标记蛋白质显示，上胚层和下胚层各组成区域的斑点数量和密度存在差异。上胚层的暗区、边缘区和中央区的蛋白质模式在定性上非常相似，但在定量上存在明显差异。然而，鸡胚盘胚胎上胚层和下胚层的任何组成区域之间的比较显示，在定性和定量上都存在差异。一种分子量为30000的蛋白质是上胚层各组成区域所特有的，而下胚层所特有的分子量为22000和37000的蛋白质很突出，似乎与原条的最初出现有关。
